感觉神经对大鼠下颌切牙釉质牙本质形成的调控作用
Sensory nerve regulates the formation of enamel and dentin in the adult rat incisor
刘安琪 1李蓓 2伍美玲 1费栋栋 3郭晓贺 1张立书 1金岩 2轩昆1
作者信息
- 1. 军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病医学研究中心,第四军医大学口腔医院儿童口腔科,陕西西安710032
- 2. 军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病医学研究中心,第四军医大学口腔医院组织病理学教研室,组织工程与再生医学研究所,陕西西安710032
- 3. 军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病医学研究中心,第四军医大学口腔医院牙周科,陕西西安710032
- 折叠
摘要
目的:研究感觉神经对大鼠下颌切牙釉质和牙本质形成的调控作用.方法:将48只成年SD大鼠随机均分为失右侧感觉神经(截断下牙槽神经)组(IANx组)、失右侧交感神经(摘除颈上神经节)组(SCGx组)、失右侧感觉神经和交感神经组(IANx+ SCGx组)、假手术组(sham组)(n=12),术后2周HE染色法观察各组大鼠的下颌切牙结构.将10只4周龄SD大鼠随机均分为IANx组和sham组(n=5),术后2周体外分离培养其牙髓干细胞(rDPSCs);经流式细胞术鉴定后,比较其克隆形成和增殖能力,并用RT-PCR检测釉质和牙本质发育相关基因Amg、Enam、Dspp、Dmp1 mRNA的表达水平.结果:IANx组和IANx+ SCGx组大鼠的右侧下颌切牙发生明显的白垩色改变,釉质和牙本质的厚度均明显低于sham组(P＜0.05).IANx组大鼠的rDPSCs与sham组相比,克隆形成和增殖能力均明显下降(P＜0.05),釉质和牙本质发育相关基因的表达水平也明显降低(P＜0.05).结论:感觉神经可通过影响rDPSCs的生物学行为而影响釉质和牙本质的形成.
Abstract
AIM:To investigate the role of sensory nerve in the formation of enamel and dentin in incisor of rats.METHODS:48 adult SD rats were divided into 4 groups (n =12) randomly.The rats received unilateral sensory nerve section (IANx),unilateral sympathetic nerve section (SCGx),unilateral sensory nerve and sympathetic nerve section (IANx + SCGx),and sham operation,respectively.The rats were sacrificed at the end of 2 W after operation and HE staining was performed to observe the changes of incisors.10 SD rats (4 W of age) were randomly divided into IANx group and sham group (n =5).rDPSCs were cultured and identified from the 2 groups of rats 2 W afte operation.The colony-forming ability,proliferation rate and expression level of genes associated with enamel and dentin formation were compared between the 2 groups.RESULTS:The incisors of IANx and IANx + SCGx groups turned chalky 2 W after denervation.HE staining showed reduced enamel and dentin formation (P ＜ 0.05).The rDPSCs of IANx group showed lower clonogenic and proliferative potential (P ＜0.05) and expressed lower levels of Amelogenin,Enamelin,Dspp and Dmp1 mRNA than sham group (P ＜0.05).CONCLUSION:Sensory nerve supports the formation of enamel and dentin by regulating rDPSCs.
关键词
感觉神经/交感神经/下牙槽神经/釉质/牙本质/牙髓干细胞Key words
sensory nerve/inferior alveolar nerve/enamel/dentin/sympathetic nerve/dental pulp stem cells引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据