静态牵张力对人炎症牙周膜干细胞成骨分化及细胞骨架重组的影响
The effects of static mechanical strain on the osteogenetic differentiation potential and cytoskeletal reorganization of human periodontal ligament stem cells derived from chronic periodontitis tissue
赵艳 1王佳帅 1郭冬会 1高洁 1何晓天 1金作林1
作者信息
- 1. 军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病 临床医学研究中心,第四军医大学口腔医院正畸科,陕西 西安 710032
- 折叠
摘要
目的:探究静态牵张力(SMS)对人炎症来源的牙周膜干细胞(PPDLSCs)的成骨分化能力及细胞骨架重组的影响.方法:体外培养PPDLSCs,用FX-T4000力学加载系统对PPDLSCs体外施加力值为12%、频率为0.1 Hz的静态牵张力,加载12 h后(对照组不加力),分别通过ALP染色、茜素红染色检测其成骨分化能力;倒置显微镜观察细胞的形态变化;免疫荧光染色和共聚焦显微镜观察细胞骨架的改变;Western blot法检测ALP、RUNX2、ROCK1、p-cofilin、Rho-GDIα、Rho-A蛋白的表达水平. 结果: 实验组PPDLSCs的矿化结节形成量明显减少;细胞骨架弹力纤维的形成受到抑制;ALP、RUNX2、ROCK1、cofilin、Rho-A蛋白表达水平下降,p-cofilin、Rho-GDIα蛋白的表达水平则升高(P<0.05). 结论:12%静态牵张力抑制PPDLSCs成骨分化和细胞骨架的重组.
Abstract
AIM:To investigate the effects of static mechanical strain(SMS) on the osteogenic differentiation potential and cytoskeletal reorganization of human periodontal ligament stem cells derived from chronic periodontitis tissue(PPDLSCs).METHODS:PPDLSCs were primarily cultured and identified.After being subjected to cyclic SMS with physiological loading of 12% at a frequency of 0.1 Hz for 12 h using a FX-4000T system,mineralization of PPDLSCs was examined by ALP staining,alizarin staining and ALP activity test.Cytoskeletal arrangement was observed by immunofluorescence staining and confocal microscopy.Protein expression was evaluated by Western blot analysis.RESULTS:SMS decreased the density of mineralized nodules and suppressed the cytoskeletal rearrangement of PPDLSCs,down-regulated ALP,RUNX2,ROCK1,cofilin and Rho-A protein expression,increased p-cofilin and Rho-GDI protein expression.CONCLUSION:SMS in the magnitude of 12% can suppress the osteogenic differentiation potential and cytoskeletal reorganization of PPDLSCs.
关键词
炎症牙周膜干细胞/细胞骨架/成骨分化/静态牵张力Key words
periodontal ligament stem cells derived from periodontitis tissue (PPDLSCs)/cytoskeleton/osteogenic differentiation/static mechanical strain (SMS)引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据