MTA诱导牙髓细胞矿化中Notch信号相关基因的表达
Expression of Notch signaling related gens in human pulp cells stimulated by MTA
郭晓睿 1关卿 1杨倩娟 2白庆霞 1常文悦 1陆群 1郭苗1
作者信息
- 1. 陕西 西安,军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学 重点实验室,第四军医大学口腔医院牙体牙髓病科,710032
- 2. 陕西 西安,西安交通大学口腔医学院,710018
- 折叠
摘要
目的:通过MTA浸提液作用于人牙髓细胞(hDPCs),探究Notch信号相关基因Notch1、Notch2及Hes1的表达变化.方法:体外培养hDPCs,用0.2 mg/mL MTA浸提液共同培养后,采用Von Kossa 染色法及RT-PCR检测其钙化结节的形成情况和不同时间点Notch信号通路相关基因Notch1、Notch2、Hes1、Runx2 mRNA的表达水平.结果:Von Kossa 染色结果显示,MTA 作用于hDPCs形成钙化结节量明显多于对照组;RT-PCR检测结果显示,Notch1、Notch2 mRNA的表达水平在MTA作用3 d时明显上升(P<0.05);Runx2 mRNA表达水平随培养时间延长而升高,7、14 d时明显高于对照组(0 h)(P<0.05);而Hes1 mRNA的表达水平则一直趋于抑制状态,从培养初期就明显低于对照组(0 h)(P<0.05).结论:Notch信号通路参与了MTA 作用下hDPCs矿化调控.
Abstract
AIM:To investigate the effects of mineral trioxide aggregate (MTA) on the expression of Notch1,Notch2 and Hes1 in human pulp cells (hDPCs).METHODS:hDPCs were in vitro cultured in the media with 0.2 mg/mL MTA,the mineralized nodule formation was observed by alizarin red staining.The mRNA expression levels of Notch1,Notch2,Hes1 and Runx2 were detected by quantitative RT-PCR.RESULTS:Cells grew normally in the media with 0.2 mg/mL MTA.MTA treatment produced a Von Kossa positive staining and the formation of mineralized nodules.The mRNA expression levels of Notch1 and Notch2 increased significantly on the 3rd day(P<0.05),then decreased gradually.The expression levels of Runx2 increased gradually over time,and on the 7th and 14th day were significantly higher than that of the control group (0 h) (P<0.05);whereas the expression of Hes1 was inbibited compared with the control group (0 h).CONCLUSION:Notch signaling may be involved in the mineralization regulation of hDPCs' stimulated by MTA.
关键词
MTA/Notch信号通路/Runx2Key words
MTA/Notch signaling/Runx2引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据