摘要
目的:采用高通量测序研究青海汉、藏族高龋患者口腔疾病微生物的多样性和群落差异.方法:选择我院口腔内科门诊汉、藏高龋患者各10例唾液样本,利用Illumina MiSeq平台进行细菌16S rRNA基因(V3-V4)区高通量测序,并利用Mothur软件分析微生物的群落结构和多样性.结果:基于97%的相似性类聚获得汉、藏高龋患者口腔微生物物种注释(Operational taxonomic unit,OTU)数目分别为51和30.汉族组分类学地位明确的有4门7纲28属,藏族组有2门4纲22属.汉族高龋组有12种优势种群,依次是:假单胞菌属(32.52%)、肠杆菌目未分类菌属(29.91%)、明串珠菌属(10.56%)、肠膜菌属(4.71%)、乳球菌属(3.71%)和链球菌属(3.04%)等.藏族组的优势菌主要有4个,依次是TM7-3未知菌属(55.86%)、未知菌属SR1(20.36%)、黄色单胞菌目未分类菌属(17.38%)和假绿黄单胞菌(2.46%).结论:青海汉族龋患者群的唾液微生物多样性与丰度明显高于藏族组,且优势微生物种群亦存在明显差异.
Abstract
AIM: To study the difference of the diversity and composition of oral microorganism between Han and Tibetan people with high caries risk in Qinghai.METHOD: 10 Han chinese and 10 Tibetans with high caries risk were selected from outpatients.High-throughput sequencing method was performed by using an Illumina miseq sequencing platform for studying bacterial 16S rRNA gene V3-V4 region,then the microbial community structure and diversity were analyzed with the Mothur software.RESULTS: Based on 97% similarity clustering,the numbers of Operational Taxonomic Units (OTUs) in Han and Tibetan groups were 51 and 30 respectively.Oral microorganisms in the Han group were classified into 4 phyla,7 classes and 36 genera,and those in the Tibetan group were classified into 2 phyla,4 classes and 27 genera.The 12 dominant species in the Han group included Pseudomonas(32.52%),Enterobacteriaceae unclassified(29.91%),Leuconostoc (10.56%),Mesenteroides(4.71%),Lactococcus(3.71%) and Streptococcus(3.04%),et al,4 dominant species in the Tibetan group were Bacteria TM7-3(55.86%),Bacteria SR1(20.36%),Xanthomonadaceae unclassified(17.38%) and Pseudomonas (2.46%).CONCLUSION: The oral microbial diversity and abundance in the Han group are significantly higher than those in the Tibetan group,there is a significant difference in the dominant oral microorganisms between the Han and Tibetan group in Qinghai region.
基金项目
青海省科技计划项目(2014ZJ759)
青海大学附属医院中青年科技计划项目(ASRF201406)
NETL
NSTL
维普
万方数据