白藜芦醇预处理提高衰老牙周膜干细胞克隆和增殖潜能的研究
Resveratrol pretreatment promotes in vitro proliferation of periodontal ligament stem cells derived from aged donors
胡超 1祝金浩 2殷园 1贺小涛 1李璇 1张曦予1
作者信息
- 1. 军事口腔医学国家重点实验室, 口腔疾病国家临床医学中心, 陕西省口腔生物工程技术研究中心, 第四军医大学口腔医院牙周科, 陕西 西安 710032
- 2. 解放军77626部队医院, 西藏 拉萨 851400
- 折叠
摘要
目的:观察白藜芦醇预处理能否衰使老牙周膜干细胞(Aged-PDLSCs)的增殖潜能和克隆形成能力得到增强.方法:将体外分离培养的中老年个体来源PDLSCs随机分为常规培养组、白藜芦醇预处理组、尼克酰胺预处理组;各组经相应处理后,采用MTT法检测其增殖潜能,结晶紫染色检测其克隆形成能力,RT-PCR和Western Blot检测其细胞中SIRT1和P53基因及蛋白的表达水平.结果:Aged-PDLSCs经白藜芦醇预处理后增殖潜能和克隆形成能力均得到了显著提高,而经尼克酰胺预处理后,其增殖潜能和克隆形成能力均明显降低;分别与常规培养组相比,差异均有统计学意义(P<0.05).此外,白藜芦醇预处理还可显著提高Aged-PDLSCs中SIRT1活性(P<0.05),显著降低了P53的表达水平(P<0.05).结论:白藜芦醇能够增强Aged-PDLSCs的克隆形成能力和增殖潜能,其作用可能是通过提高SIRT1的表达水平而实现的.
Abstract
AIM: To investigate whether resveratrol(AV) can promote the proliferation and colony-forming of periodontal ligament stem cells (PDLSCs) derived from aged donors.METHODS: The PDLSCs were acquired from the periodontal ligament tissues of middle-aged and elderly subjects by enzymolysis and cultured in vitro.the PDLSCs were not pretreated(control group) or pretreated by AV (AV group) or nicotinamide(NT group).Cell proliferation was determined by MTT assay and colony formation was detected by crystal violet staining.PCR and Western blotting were performed to determine the relative expressions of SIRT1 and HIF1-α.RESULTS: AV pretreatment enhanced the capacities of proliferation and colony formation of PDLSCs derived from aged donors (P<0.05),while NT impaired the proliferation and colony formation of the PDLSCs (P<0.05).The expression of SIRT1 was promoted and the expression of P53 was down-regulated by AV pretreatment (P<0.05).CONCLUSION: Resveratrol can reverse the proliferation and colony formation of PDLSCs derived from aged donors via the activation of SIRT1.
关键词
牙周膜干细胞/白藜芦醇/尼克酰胺/衰老/SIRT1Key words
periodontal ligament stem cells/resveratrol/nicotinamide/senescence/SIRT1引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据