转化生长因子β1对牙周膜干细胞成骨分化影响的实验研究
The effects of TGF-β1 on the osteogenic differentiation of periodontal ligament stem cells
丁欣欣 1李淑慧 1马玉 1尹艳娇 1葛翘诚 1谢超 1王雪 1吴佩玲1
作者信息
- 1. 新疆医科大学第二附属医院口腔科, 新疆 乌鲁木齐 830063
- 折叠
摘要
目的:探讨转化生长因子β1(TGF-β1)对人牙周膜干细胞(hPDLSCs)增殖和骨向分化的影响. 方法:将体外培养的hPDLSCs随机分为对照组(不含TGF-β1)和2个实验组(1、10 ng/mL TGF-β1),用CCK8检测细胞的增殖情况;碱性磷酸酶(ALP)活性、茜素红染色及实时定量PCR检测各组细胞的成骨分化情况.结果:TGF-β1可促进细胞增殖,且10 ng/mL组细胞的增殖能力较强(P<0.05);与对照组相比,实验组的ALP活性明显升高,且10 ng/mL 组较1 ng/mL组升高更明显(P<0.05);茜素红染色结果显示,实验组hPDLSCs染色显著,矿化结节形成范围大且呈深橘色,10 ng/mL组较1 ng/mL组染色更显著;RT-PCR结果显示,与对照组相比,10 ng/mL组成骨相关基因牙骨质附着蛋白(CAP)、骨桥蛋白(OPN)、骨钙蛋白(OCN)、Runt相关转录因子2(Runx2)表达量明显升高( P<0.05).结论:10 ng/mL的TGF-β1可明显促进hPDLSCs的骨向分化.
Abstract
AIM:To evaluate the effects of TGF-β1 on the osteogenesis of human periodontal ligament stem cells (hPDLSCs).METHODS:hPDLSCs were in vitro cultured with TGF-β1 at 0 ng/mL (control group), 1 ng/mL and 10 ng/mL respectively.CCK8 assay, alkaline phosphatase(ALP) activity assay, alizarin red staining and real time quantitative PCR were conducted for the evaluation of the osteogenesis of hPDLSCs.RESULTS:TGF-β1 promoted cell proliferation, and 10 ng/mL TGF-β1 was the most effective.ALP in the experimental groups was higher than in the control group, in 10 ng/mL group was higher than in 1 ng/mL group (P<0.05).Mineralized nodule formation in the experimental groups was significantly more than in the control group, and in 10 ng/mL group was more than in 1 ng/mL group.The mRNA expression of osteopontin(OPN), osteocalcin(OCN), Runt-related transcription factor 2(RUNX-2) and Cementum Attachment Protein(CAP) were significantly increased in 10 ng/mL TGF-β1 group(P<0.05).CONCLUSION:TGF-β1 is a potent regulator of osteogenesis differentiation of human periodonal ligament cells.
关键词
牙周膜干细胞/转化生长因子-β1/骨向分化Key words
periodontal ligament stem cells/TGF-β1/osteogenesis引用本文复制引用
基金项目
新疆维吾尔自治区高校科研计划青年教师科研启动基金(XJEDC2016S055)
出版年
2017
NETL
NSTL
维普
万方数据