摘要
目的:在微弧氧化钛表面构建壳聚糖(CS)/antimiR-138涂层并研究其生物学性能.方法:用CS包裹antimiR-138形成CS/antimiR-138载体颗粒,通过硅烷偶联的方法将其加载至经微弧氧化(MAO)处理的钛表面.再将钛试件分为MAO表面加载CS/antimiR-138组、加载CS/antimiR-control组、加载硅烷偶联处理组(MAO-SGC)和未经MAO处理组(MAO)(n=5),用接触角测量仪测量其表面水接触角;SEM观察其表面微形貌;CCK-8分析其生物相容性;RT-PCR检测其对MSCs内miR-138及成骨相关基因表达的影响.结果:各组钛表面的水接触角为MAO组<MAO-SGC组<MAO-CS/miRNA组;SEM观察表明MAO-SGC组表面及孔洞内部均有一层膜状物质,表面较平坦,局部微突起,加载CS/miRNA后其表面孔洞的内部及周围微结构较粗糙,可见大面积的颗粒状物质沉积于表面;CCK-8测定结果表明,4组材料生物相容性无统计学差异(P>0.05);RT-PCR 结果表明,MAO-CS/antimiR-138组表面细胞内miR-138的表达量明显低于其他3组(P<0.05),各成骨相关基因的表达量明显高于其他3组(P<0.05).结论:在微弧氧化钛表面构建CS/antimiR-138涂层,生物相容性优良,可促进MSCs的成骨分化.
Abstract
AIM:To fabricate CS/antimiR-138 modified microporous surface on Ti implants.METHODS:The CS/antimiR-138 complexes were fabricated, and then loaded onto the MAO Ti surface by silane glutaraldehyde coupling.The MAO titanium surface was respectively loaded by CS/antimiR-138, CS/antimiR-control,4 silane glutaraldehyde coupling agent (MAO-SGC) and the naked MAO(MAO)(n=5).Water contact angle was measured.SEM was used to observe the surface morphology.Bone marrow mesenchymal stem cells(MSCs)were cultured on the specimens.The cell viability was examined by CCK-8 assay.The expressions of miR-138 and osteogenesis-related genes of the cells were evaluated by real-time PCR.RESULTS:The water contact angles of MAO, MAO-SGC and MAO-CS/antimiR-138 groups increased in turn significantly.The cell viability was not significantly different among the groups(P>0.05).MAO-CS/antimiR-138 group exhibited lower expression of miR-138(P<0.05) and higher expression of the osteogenesis-related genes than the other 3 groups(P<0.05).CONCLUSION:Covalent bonding of CS/antimiR-138 onto MAO titanium surface can improve osteogenic differentiation of MSCs without cytotoxicity.
基金项目
国家青年科学基金(31500776)
青岛市科技局自主创新计划(15-9-1-23-jch)
NETL
NSTL
维普
万方数据