GDF11抑制老龄鼠骨形成的实验研究
GDF11 inhibits bone formation of aged mice: an experimental study
朱彪 1焦婷 2赵刚 3张佳佳 4郭希民 5郭红延 5向光大1
作者信息
- 1. 中国人民解放军武汉总医院内分泌与代谢科,湖北武汉430070;南方医科大学研究生院,广东广州510515
- 2. 南方医科大学研究生院,广东广州510515;河北大学附属医院肿瘤科,河北保定071000
- 3. 南方医科大学研究生院,广东广州510515
- 4. 中国人民解放军武汉总医院内分泌与代谢科,湖北武汉430070
- 5. 南方医科大学研究生院,广东广州510515;武警总医院口腔医学中心,北京100039
- 折叠
摘要
目的:探讨生长分化因子11(GDF11)对老龄鼠骨代谢的影响.方法:将20只18月龄C57BL/6J小鼠随机分为实验组(GDF11组)和对照组(Vehi组)(n=10),GDF11组按每天0.3 mg/kg的剂量腹腔注射GDF11,Vehi组注射等量生理盐水;连续注射4周后,分别检测其血、尿中骨代谢相关生化指标,并用Mi-cro CT分析股骨的形态学指标.然后再取C57BL/6J小鼠的P1代骨髓间充质干细胞(BMSCs),并将其随机分为2组,分别用含GDF11为0(对照组)、30 g/mL(实验组)诱导培养3d后,qRT-PCR检测Runx2、PPAR-¨COL-1、OCN等成骨、成脂相关基因的表达水平.结果:GDF11干预可显著降低小鼠骨合成代谢的生化指标,并加重其股骨的骨质疏松(P＜0.05).体外实验结果显示,GDF11干预可显著降低小鼠BMSCs中Runx2、COL-1基因的表达水平(P＜0.05),并显著上调其成脂相关基因PPAR-y的表达水平(P＜0.05).结论:GDF11能够抑制老龄小鼠的骨形成.
Abstract
AIM:To investigate the effect of GDF11 on bone metabolism of aged mice.METHODS:Twenty 18-month old C57 mice were randomly divided into GDF11 group (0.3 mg · kg-1 · d-1 intraperitoneal injection,n =10) and Vehi group (equivalent physiological saline,n =10),4 weeks after intervention,biochemical marks of bone metabolism were tested and histomorphometric indexes of femurs were measured by microcomputed tomography.RESULTS:GDF11 decreased biochemical marks of bone formation including COL-1 and OCN;increased the expression of PPAR-γ;enhanced the histomorphologic indexes of osteoporosis of femurs.CONCLUSION:GDF11 inhibits bone formation of old mice.
关键词
GDF11/骨髓间充质干细胞/成骨细胞/骨质疏松Key words
GDF11/BMSCs/osteoblast/osteoporosis引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据