摘要
目的:研究表没食子儿茶素浸食子酸酯(EGCG)对变异链球菌和乳酸杆菌生长的抑制作用,以及最低抑菌浓度(MIC)下EGCG溶液促进脱矿釉质再矿化的作用.方法:采用牛津杯法进行抑菌实验,测定EGCG对变异链球菌和乳酸杆菌的MIC.然后再将化学方法制备的釉质脱矿模型随机分为3组,并分别以MIC浓度的EGCG溶液、等浓度的NaF溶液(阳性对照)、蒸馏水(空白对照)为实验溶液,用pH循环法对相应组脱矿釉质进行再矿化;同时用维氏硬度仪测定各标本的表面硬度,扫描电镜观察其表面形貌.结果:EGCG对变异链球菌和乳酸杆菌均有抑制作用,其MIC为0.25 mg/mL.再矿化处理后,各组釉质的显微硬度以NaF组最高,EGCG组次之,空白对照组最低,组间两两相比均有统计学差异(P＜0.05);扫描电镜观察还发现,EGCG和NaF再矿化组的釉质表面均有颗粒状沉积物,且表面趋于平整.结论:EGCG不仅可以抑制致龋菌,还可以促进釉质的再矿化.
Abstract
AIM:To investigate the effect of green tea extracts epigallocatechin gallate (EGCG) on the growth of Streptococcus mutans and lactobacillus.And to investigate the efficacy of EGCG solution on remineralization of etched enamel surface.METHODS:The antibacterial experiment was carried out by Oxford cup method,examination of the minimal inhibitory concentration (MIC) of EGCG against Streptococcus pneumoniae and lactobacillus.EGCG with MIC was ued for the remineralization of enamel surface etched by pH circulation method.The same concentration of NaF solution was used as positive control,distilled water as blank control.RESULTS:The MIC of EGCG on streptococcus mutans and lactobacilli was 0.25 mg/mL.And the remineralization ability of mixed solution of EGCG of MIC on etched enamel was obvious.CONCLUSION:EGCG can restrain cariogenic bacteria and promote enamel remineralization.
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维普
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