氯己定凝胶和氢氧化钙糊剂抑菌作用的对比研究
In vitro evaluation of the bacteriostatic effects of chlorhexidine gel and calcium hydroxide
李韶容 1刘颖 2杨子 1侯本祥1
作者信息
- 1. 首都医科大学附属北京口腔医院牙体牙髓科 北京100050
- 2. 首都医科大学附属北京口腔医院研究所 北京100050
- 折叠
摘要
目的:研究氯己定凝胶(CHX)、氢氧化钙糊剂(CH)及其混合物(CHX +CH)对粪肠球菌、牙龈卟啉单胞菌、伴放线放线杆菌、微小小单胞菌的抑菌作用.方法:采用琼脂扩散法测量CHX、CH及CHX+CH的抑菌圈;将以上4种细菌悬液分别稀释成1.5×108、3 × 108、6×108、9×108、12×108、15×108 CFU/mL不同浓度,通过直接接触法比较各药物的抑菌效果,并用Kruskal-Wallis检验进行统计分析.结果:抑菌圈从大到小依次为:CHX、CHX+ CH、CH(各药物间相比,P＜0.05);对粪肠球菌的抑制效果为:CHX+ CH＞ CHX＞ CH,而对牙龈卟啉单胞菌、伴放线放线杆菌及微小小单胞菌的抑菌效果为:CHX＞ CH＞CHX(各药物间相比,P＜0.05).结论:氯己定凝胶扩散作用最强,氯己定凝胶和氢氧化钙糊剂混合物直接接触抑菌作用最强.
Abstract
AIM:To evaluate the antibacterial activity of chlorhexidine (CHX) gel and/or calcium hydroxide (CH) paste against endodontic pathogens.METHODS:Agar diffusion test and direct contact test were used to obtain the inhibition zone and the minimum inhibition concentration (MIC) of CHX,CH and CHX + CH against 4 endodontic pathogens.Data were statistically analyzed using Kruskal-Wallis test.RESULTS:The diameter of the inhibition zone of the preparations was as following:CHX ＞ CHX + CH ＞ CH(P ＜0.05).CHX + CH showed the strongest inhibition effect for all the pathogens.In the inhibition of enterococcus faecalis the effeciency of the preparations was CHX + CH ＞ CHX ＞ CH(P ＜ 0.05);in the inhibition of Porphyromonas gingivalis,Actinobacillus actinomycetem comitans and Parvimonasmicra CHX ＞ CH ＞ CHX(P ＜ 0.05).CONCLUSION:The diffusion ability of CHX gel was the largest while the direct antibacterial activity of CHX + CH was the strongest against the 4 endodontic pathogens.
关键词
氯己定凝胶(CHX)/氢氧化钙糊剂(CH)/粪肠球菌/微小小单胞菌/抑菌活性Key words
chlorhexidine gel (CHX)/calcium hydroxide paste (CH)/enterococcus faecalis/parvimonasmicra/bacteriostasis引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据