The regulatory role of chemokine SDF-1 on macrophage migration and polarization
AIM:To investigate the regulatory role of the chemokine SDF-1(Stromal cell-derived factor-1)and its receptors CXCR4(C-X-C chemokine receptor type4)and CXCR7(C-X-C Chemokine receptor type 7)on macrophage migration and polarization.METHODS:The effect of the SDF-1/CXCR4 and SDF-1/CXCR7 pathways on macrophage migration was assessed using Transwell assays;the influence of SDF-1 on the expression of CXCR4 and CXCR7 receptor proteins was examined by Western blot analysis;and the impact of SDF-1/CXCR4 and SDF-1/CX-CR7 pathways on the regulation of macrophage polarization was measured using RT-qPCR,ELISA,and flow cytome-try.RESULTS:SDF-1 significantly promoted macrophage migration and enhanced the expression of CXCR4 and CX-CR7 receptor proteins(P＜0.001).The migration-promoting effect of SDF-1 could be inhibited by AMD3100 and CX-CR7 antagonist-1(P＜0.001).SDF-1 also increased the expression of M1 markers TNF-α,iNOS,and M2 markers IL-10,TGF-β(P＜0.05),while CXCR7 antagonist-1 decreased the expression of these markers(P＜0.01).AMD3100 had no significant effect on SDF-1-induced enhancement of marker expressions(P＞0.05).Flow cytometry results indicated that compared to the control group,SDF-1 significantly increased the proportion of M2 type expres-sion,and this effect could be suppressed by CXCR7 antagonist-1.CONCLUSION:SDF-1 can activate the CXCR4/CXCR7 signaling pathway,and its macrophage migration-promoting effects are achieved through both CXCR4 and CX-CR7 receptors,while the regulation of macrophage polarization is mainly mediated by the CXCR7 receptor.
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