Preparation and performance evaluation of injectable molybdenum/diatom biosilica/chitosan bone filling material
AIM:Construction and evaluation of an injectable slow-release molybdenum(Mo)bone filling material.METHODS:Diatom biosilica(DBs)was loaded with different ratios of Mo ions,then blended with chitosan(CS)-based hydrogel to build the Mo/DBs/CS slow-release system.The physical and chemical properties were exam-ined by the scanning electron microscope(SEM),the energy spectrometer(EDS),the fourier infrared spectrum de-vice(FTIR)and the inductively coupled plasma mass spectrometer(ICP).The biological compatibility and the ability of promoting bone differentiation of rat bone marrow stem cells were observed by cytotoxic experiment,the level of alka-line phosphatase(ALP)secretion,the ALP and Alizarin red staining.RESULTS:SEM,EDS and FTIR test results showed that Mo ions can be successfully loaded in DBs.The encapsulation rate of Mo by DBs was around 8％.At 21d,the total release rate of the Mo ion in Mo/DBs/CS group was 81％,which had the best slow release effect.Mo ion un-der 200 mg/L and the DBs leaching liquid under 2 500 mg/L were not cytotoxic to BMSCs.Mo ion concentration at 25～3.13 mg/L could increase the secreting of ALP by BMSCs(P＜0.001).ALP and Alizarin red staining results showed that Mo/DBs/CS system significantly promote the osteogenesis differentiation of BMSCs.CONCLUSION:The Mo/DBs/CS slow-release system is a kind of bone defect filling material with good biological compatibility and osteoin-ductive activity.
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