MiR-205-3p targets PRMT5 to improve inflammatory response and apoptosis of HUVECs induced by P.g-LPS
AIM:To investigate the effect of miR-205-3p on the inflammatory response and apoptosis of HU-VECs induced by P.g-LPS and its regulatory mechanism.METHODS:HUVECs were stimulated with P.g-LPS to estab-lish a cell injury model.The experiment was divided into Con group,LPS group,LPS+miR-NC group,LPS+miR-205 group,LPS+si-NC group,LPS+si-PRMT5 group,LPS+miR-205+pc-NC group,and LPS+miR-205+pc-PRMT5 group.RT-qPCR and Western blot were used to detect the expression of miR-205-3p,PRMT5,and apoptosis proteins.ELISA was used to detect the expression of inflammatory factors.Tunel staining and flow cytometry were used to detect HUVECs apoptosis.The dual luciferase reporter gene assay and Western blot were used to verify the targeted relationship between miR-205-3p and PRMT5.RESULTS:After P.g-LPS stimulation of HUVECs,the expression of inflammatory factors and apoptosis rate significantly increased,while miR-205-3p was down-regulated and PRMT5 was up-regulated(P＜0.05).miR-205-3p can inhibit PRMT5 expression,and overexpression of miR-205-3p and low expression of PRMT5 can reduce inflammatory factor expression and apoptosis rate(P＜0.05).The results of the dual luciferase reporter gene assay and West-ern blot showed that miR-205-3p can target and inhibit the expression of PRMT5(P＜0.05).Overexpression of PRMT5 can significantly reverse the inhibitory effect of overexpression of miR-205-3p on inflammatory response and apoptosis(P＜0.05).CONCLUSION:P.g-LPS promotes HUVECs inflammatory response and apoptosis;miR-205-3p targets and negatively regulates PRMT5 expression to improve P.g-LPS-induced HUVECs inflammatory response and apoptosis.
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