RGD-loaded PLA-HA bone biomimetic scaffold for promoting early bone defect repair
AIM:To investigate osteogenic properties of scaffolds in rat femoral defects by modifying biomimetic scaffolds with the Arginine-Glycine-Aspartic acid(RGD).METHODS:GelMA and RGD were modified in poly(lactic acid)-hydroxyapatite(PLA-HA)scaffolds prepared by three-dimensional(3D)printing to construct composite hydrogel scaffolds PLA-HA/GelMA/RGD(PHD).The surface morphology of PHD scaffold was observed by scanning electron microscope(SEM),and the proliferation of bone marrow mesenchymal stem cells(BMSCs)was assessed by CCK-8 assay.18 SD rats were randomly divided into three groups(n=6)and implanted with PLA-HA/GelMA(PHG)and PHD scaffolds,while no implantation was performed in the blank control group.At 4 weeks,the bone defect area and new bone index were measured by micro-CT,and the femoral samples in the defect area were stained with H&E staining as well as Masson trichrome staining to observe the histological morphology.RESULTS:SEM images indicated that the pore sizes of the scaffolds were distinct,and GelMA was stably attached to the surface of the scaffolds.CCK-8 results demonstrated the cell proliferation in the PHD group was superior compared to PHG group.At 4 weeks after surgery,the 3D reconstructed micro-CT images revealed that abundant new bone was observed in the PHD group compared to the blank group and PHG group.Simultaneously,quantitative analysis indicated bone volume fraction and bone trabecular thickness were significantly enhanced in PHD group.Histological staining showed that the PHD group formed continuous and compact bone tissue.CONCLUSION:The RGD-loaded PLA-HA bone biomimetic scaffolds can facilitate osteoblast proliferation in vitro and induce new bone formation in the rat femur defect model,thereby successfully achieving early bone defect repair.
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