In vitro study of the effect of final rinse solution on dentin tubule permeability of root canal sealer
AIM:This in vitro study aimed to observe the tubular penetration ability of two different sealers in apical,middle and coronal after used with different final irrigation solutions.METHODS:Seventy-two single-rooted premolar teeth were collected.The crown was sectioned,and the canals were prepared with TF rotary instruments and irrigated with sodium hypochlorite(NaClO)and ethylenediaminetetraacetic acid(EDTA).The samples were divided into 6 groups according to the final irrigation used:Octenisept®;2%Chlorhexidine(CHX);5.25%NaClO;2.5%NaClO;1%NaClO;saline(control).Then,they were divided into two groups according to different sealers:iRoot SP;AH-Plus.The mean maximum depth and total percentage of sealer penetration were measured using confocal laser scanning microscope.RESULT:All samples exhibited the tubular penetration ability of root canal sealers in apical,middle and coronal.Significant differences were observed in the permeability distribution(penetration distance,penetration area)among these three regions(P<0.001).In terms of the deepest tubular penetration distance,regardless of the type of root canal sealer,the 5.25%NaClO group was significantly higher than the NS group in the coronal and middle segments(P<0.05);in the apical segment,the NaClO group was higher than the other groups(P<0.05).In terms of the average tubular permeation area,in all root canal segments,when the sealer was iRoot SP,the high concentration NaClO group(5.25%,2.5%)was significantly larger than the other groups(P<0.05);When the sealing agent was AH Plus,The high concentration NaClO group has well permeability of dentinal tubules(P<0.05).CONCLUSION:5.25%NaClO group has well permeability of dentinal tubules;The root canal sealer iRoot SP showed better tubules permeability than AH-Plus.the combination of 5.25%NaClO and iRoot SP is superior to other combinations.
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