摘要
目的:比较终末冲洗液(Octenisept®、2%Chlorhexidine、5.25%NaClO、2.5%NaClO、1%NaClO、Saline)对两种根管封闭剂(iRoot SP、AH-Plus)的牙本质小管渗透性的影响.探讨最佳的终末冲洗液和根管封闭剂配伍.方法:收集因正畸拔除的单根管前磨牙72个,截冠标准化牙根长度为(14±0.5)mm,用TF镍钛系统根管预备.根据不同终末冲洗液随机分为6组(n=12)进行终末冲洗,A组Octenisept®,B组 2%Chlorhexidine,C组5.25%NaClO,D组2.5%NaClO,E组1%NaClO,F组Saline.充填时,根据不同的根管封闭剂再分为2个亚组(n=6),1组 iRoot SP,2组AH-Plus,封闭剂染色,单尖法充填根管.距离根尖2 mm,5 mm,8 mm处各制备0.1 mm厚的切片,置于共聚焦激光显微镜下观察,分析并测量封闭剂的牙本质小管平均最大渗透深度和渗透面积比.结果:所有样本根冠、中、尖三个部位均能观察到根管封闭剂渗入牙本质小管.3个部位之间的渗透性分布(渗透距离、渗透面积)具有显著差异(P<0.001).牙本质小管最深渗透距离方面,无论是何种根管封闭剂,在根冠部和根中部,5.25%NaClO组显著高于NS组(P<0.05);在根尖部,NaClO组均高于其余组(P<0.05).牙本质小管平均渗透面积方面,在所有根管段,封闭剂为iRoot SP时,高浓度NaClO组(5.25%、2.5%)显著大于其余组(P<0.05);封闭剂为AH-Plus时,在所有根管段,高浓度NaClO组显著高于NS组(P<0.05).结论:高浓度NaClO终末冲洗的牙本质小管渗透表现较佳;封闭剂iRoot SP的牙本质小管渗透面积稍优于AH-Plus;5.25%NaClO终末冲洗配合iRoot SP渗透性效果优于其它组合.
Abstract
AIM:This in vitro study aimed to observe the tubular penetration ability of two different sealers in apical,middle and coronal after used with different final irrigation solutions.METHODS:Seventy-two single-rooted premolar teeth were collected.The crown was sectioned,and the canals were prepared with TF rotary instruments and irrigated with sodium hypochlorite(NaClO)and ethylenediaminetetraacetic acid(EDTA).The samples were divided into 6 groups according to the final irrigation used:Octenisept®;2%Chlorhexidine(CHX);5.25%NaClO;2.5%NaClO;1%NaClO;saline(control).Then,they were divided into two groups according to different sealers:iRoot SP;AH-Plus.The mean maximum depth and total percentage of sealer penetration were measured using confocal laser scanning microscope.RESULT:All samples exhibited the tubular penetration ability of root canal sealers in apical,middle and coronal.Significant differences were observed in the permeability distribution(penetration distance,penetration area)among these three regions(P<0.001).In terms of the deepest tubular penetration distance,regardless of the type of root canal sealer,the 5.25%NaClO group was significantly higher than the NS group in the coronal and middle segments(P<0.05);in the apical segment,the NaClO group was higher than the other groups(P<0.05).In terms of the average tubular permeation area,in all root canal segments,when the sealer was iRoot SP,the high concentration NaClO group(5.25%,2.5%)was significantly larger than the other groups(P<0.05);When the sealing agent was AH Plus,The high concentration NaClO group has well permeability of dentinal tubules(P<0.05).CONCLUSION:5.25%NaClO group has well permeability of dentinal tubules;The root canal sealer iRoot SP showed better tubules permeability than AH-Plus.the combination of 5.25%NaClO and iRoot SP is superior to other combinations.
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