Chemical derivatization strategies for enhancing the HPLC analytical performance of natural active triterpenoids

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国家科技期刊平台
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外文摘要：Triterpenoids widely exist in nature,displaying a variety of pharmacological activities.Determining triterpenoids in different matrices,especially in biological samples holds great significance.High-performance liquid chromatography(HPLC)has become the predominant method for triterpenoids analysis due to its exceptional analytical performance.However,due to the structural similarities among botanical samples,achieving effective separation of each triterpenoid proves challenging,necessitating significant improvements in analytical methods.Additionally,triterpenoids are characterized by a lack of ultraviolet(UV)absorption groups and chromophores,along with low ionization efficiency in mass spectrometry.Consequently,routine HPLC analysis suffers from poor sensitivity.Chemical derivatization emerges as an indispensable technique in HPLC analysis to enhance its performance.Considering the structural characteristics of triterpenoids,various derivatization reagents such as acid chlorides,rho-damines,isocyanates,sulfonic esters,and amines have been employed for the derivatization analysis of triterpenoids.This review comprehensively summarized the research progress made in derivatization strategies for HPLC detection of triterpenoids.Moreover,the limitations and challenges encountered in previous studies are discussed,and future research directions are proposed to develop more effective derivatization methods.

外文关键词：

TriterpenoidHPLCDerivatizationQuantification

作者：

Xiao-Feng Huang、Ying Xue、Li Yong、Tian-Tian Wang、Pei Luo、Lin-Sen Qing

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作者单位：

Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China

University of Chinese Academy of Sciences,Beijing 100049,China

Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,610041,China

Sichuan Center for Disease Control and Prevention,Chengdu,610041,China

Sichuan Center for Disease Control and Prevention,Chengdu 610041,China

State Key Laboratory for Quality Research in Chinese Medicines,Macau University of Science and Technology,Macao,China

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出版年：

2024

DOI：

10.1016/j.jpha.2023.07.004

药物分析学报(英文)

西安交通大学

药物分析学报(英文)

影响因子：0.244

ISSN：2095-1779

年,卷(期)：2024.14(3)