首页|Altered synaptic currents,mitophagy,mitochondrial dynamics in Alzheimer's disease models and therapeutic potential of Dengzhan Shengmai capsules intervention

Altered synaptic currents,mitophagy,mitochondrial dynamics in Alzheimer's disease models and therapeutic potential of Dengzhan Shengmai capsules intervention

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Emerging research suggests a potential association of progression of Alzheimer's disease(AD)with al-terations in synaptic currents and mitochondrial dynamics.However,the specific associations between these pathological changes remain unclear.In this study,we utilized Aβ42-induced AD rats and primary neural cells as in vivo and in vitro models.The investigations included behavioural tests,brain magnetic resonance imaging(MRI),liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)analysis,Nissl staining,thioflavin-S staining,enzyme-linked immunosorbent assay,Golgi-Cox staining,trans-mission electron microscopy(TEM),immunofluorescence staining,proteomics,adenosine triphosphate(ATP)detection,mitochondrial membrane potential(MMP)and reactive oxygen species(ROS)assess-ment,mitochondrial morphology analysis,electrophysiological studies,Western blotting,and molecular docking.The results revealed changes in synaptic currents,mitophagy,and mitochondrial dynamics in the AD models.Remarkably,intervention with Dengzhan Shengmai(DZSM)capsules emerged as a pivotal element in this investigation.Aβ42-induced synaptic dysfunction was significantly mitigated by DZSM intervention,which notably amplified the frequency and amplitude of synaptic transmission.The cognitive impairment observed in AD rats was ameliorated and accompanied by robust protection against structural damage in key brain regions,including the hippocampal CA3,primary cingular cortex,prelimbic system,and dysgranular insular cortex.DZSM intervention led to increased IDE levels,augmented long-term potential(LTP)amplitude,and enhanced dendritic spine density and length.Moreover,DZSM intervention led to favourable changes in mitochondrial parameters,including ROS expression,MMP and ATP contents,and mitochondrial morphology.In conclusion,our findings delved into the realm of altered synaptic currents,mitophagy,and mitochondrial dynamics in AD,concurrently highlighting the therapeutic potential of DZSM intervention.

Alzheimer's diseaseSynaptic currentsMitophagyMitochondrial fusion and fissionDengzhan Shengmai capsules

Binbin Zhao、Dongfeng Wei、Qinghua Long、Qingjie Chen、Fushun Wang、Linlin Chen、Zefei Li、Tong Li、Tao Ma、Wei Liu、Linshuang Wang、Caishui Yang、Xiaxia Zhang、Ping Wang、Zhanjun Zhang

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State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research,Beijing Normal University,Beijing,100875,China

Institute of Gerontology,Hubei University of Chinese Medicine,Wuhan,430065,China

Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China

Medical School,Hubei Minzu University,Enshi,Hubei,445000,China

Hubei University of Science and Technology,Xianning,Hubei 437100,China

Institute of Brain and Psychological Science,Sichuan Normal University,Chengdu 610066,China

Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription,Ministry of Education,Hubei University of Chinese Medicine,Wuhan 430065,China

Institute of Gerontology,Hubei University of Chinese Medicine,Wuhan 430065,China

Dongfang Hospital,Beijing University of Chinese Medicine,Beijing,100078,China

Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases,Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing,100700,China

School of Systems Science,Beijing Normal University,Beijing,100875,China

BABRI Centre,Beijing Normal University,Beijing,100875,China

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2024

10.1016/j.jpha.2023.10.006

药物分析学报(英文)
西安交通大学

药物分析学报(英文)

影响因子：0.244
ISSN：2095-1779
年,卷(期)：2024.14(3)