首页|Signal interference between drugs and metabolites in LC-ESI-MS quantitative analysis and its evaluation strategy
Signal interference between drugs and metabolites in LC-ESI-MS quantitative analysis and its evaluation strategy
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国家科技期刊平台
NETL
NSTL
万方数据
Liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS)is a widely utilized technique for in vivo pharmaceutical analysis.Ionization interference within electrospray ion source,occurring between drugs and metabolites,can lead to signal variations,potentially compromising quantitative accuracy.Currently,method validation often overlooks this type of signal interference,which may result in systematic errors in quantitative results without matrix-matched calibration.In this study,we conducted an investigation using ten different groups of drugs and their corresponding me-tabolites across three LC-ESI-MS systems to assess the prevalence of signal interference.Such in-terferences can potentially cause or enhance nonlinearity in the calibration curves of drugs and metabolites,thereby altering the relationship between analyte response and concentration for quanti-fication.Finally,we established an evaluation scheme through a step-by-step dilution assay and employed three resolution methods:chromatographic separation,dilution,and stable labeled isotope internal standards correction.The above strategies were integrated into the method establishment process to improve quantitative accuracy.
Liquid chromatography-electrosprayionization tandem mass spectrometry(LC-ESI-MS)DrugsMetabolitesIonization interferenceQuantitative analysis
国家科技期刊平台
NSTL
万方数据
