Objective To understand the pathogen composition and changing trend of viral diarrhea in children under 5 years old in Lulong County,Hebei Province,so as to provide scientific basis for the prevention and treatment of viral diarrhea in infants and young children.Methods A total of 832 stool samples of children under 5 years old were collected from a Lulong County hospital and maternal and child health hospital from January 1,2016 to December 31,2021,and the epidemiological case study form was filled in.Rotavirus(RV)antigen and its positive G/P samples were further typing by enzyme linked immunosorbent assay(ELISA)and reverse transcription PCR(RT-PCR),respectively.Human calicivirus(HuCV),enteric Adenovirus(EAdV)and Astrovirus(AstV)were detected by RT-PCR or real-time quantitative PCR.Norovirus(NV)positive samples were amplified by RT-PCR and then sequenced and typed.Results A total of 576 of 832 samples were positive for four kinds of viruses,and the total positive detection rate was 69.23％.The positive detection rates of RV,HuCV,EAdV and AstV were 38.22％,31.01％,6.01％and 5.17％,respectively.Single pathogen infection detection rate was 58.41％,and mixed infection detection rate was 10.82％,of which 87 samples were co-infected with two viruses and 3 samples were co-infected with three viruses.There was no statistically significant difference in the positive detection rates of viruses and mixed infections between different genders(x2rV=0.811,PRV=0.368;XHuCV=0.286,PHuCV=0.593;x2EAdV=0.156,PEAdV=0.693;x2AstV=0.035,PAstV=0.852;x2edinfection=0.617,Pmixed infection=0.432).RV and HuCV had the highest positive detection rates of 47.69％and 33.85％at the age of 18 to less than 24 months(X2V=18.748,PRV=0.009;x2HuCV=2.797,PHuCV=0.903),respectively.The peak seasons of viral diarrhea were January to March,November and December.RV had the highest positive detection rate in February(59.51％),and HuCV had the highest positive detection rate in November(62.90％).HuCV and EAdV diarrhea occurred all year round.The proportion of type G9P[8]in RV was 90.25％,and the proportion of type GⅡ in HuCV was 67.05％.Conclusion RV and HuCV are the main pathogens of viral diarrhea in infants and young children under 5 years old in Lulong County,and the positive detection rate of HuCV increased greatly.RV type G9P[8]and HuCV type G Ⅱ are the main epidemic strains.
Lulong CountyInfants and young childrenViral diarrheaEtiologic analysis
NETL
NSTL
万方数据
