Research on the determination of urinary iodine based on high matrix introduction-inductively coupled plasma mass spectrometry
Objective To establish a method for the analysis of urinary iodine by high matrix introduction-inductively coupled plasma mass spectrometry(HMI-ICP-MS)and apply to the clinical detection of urinary iodine,to understand the urinary iodine intake of children in Guangzhou City in 2019,and to provide a scientific basis for the development of iodine deficiency disease prevention and control measures.Methods The optimal HMI condition was obtained by adjusting the flow rate of atomization gas,dilution gas and reaction gas.The detection limit,precision and accuracy were measured to evaluate the method of determination of urinary iodine by HMI-ICP-MS.The urinary iodine of children in Guangzhou was statistically analyzed by the non-parametric rank sum test.Results When atomizing gas flow rate was 0.4ml/min,diluting gas flow rate was 0.7ml/min,collision gas flow rate was 4.4ml/min,HMI could obtain the best working conditions.In the range of 1.0-40.0μg/L of urineiodine concentration,the linearity of the method was good,and the correlation coefficient was 0.999 9-1.000 0.The detection limit was 0.088μg/L determined by a 3-fold signal-to-noise ratio(S/N=3).The spiked recovery rate of urinary iodine was 71.3％-101.3％,with a relative standard deviation of 1.2％-2.4％.In 2019,the median urinary iodine concentration of 577 boys aged 8-10 years in Guangzhou City was 203.4μg/L,and the median urinary iodine concentration of 566 girls was 197.0μg/L.There was no significant difference in urinary iodine levels between children of different genders(Z=20.703,P=0.931).Conclusion The HIM-ICP-MS method has high accuracy and good precision meeting the methodological requirements.This method has a higher ability to tolerate the detection of a large number of high-salt samples.Gender itself has little impact on urinary iodine levels.
High matrix introductionIodine deficiency diseaseInductively coupled plasma mass spectrometryUrinary iodineChildren
NETL
NSTL
万方数据
