摘要
目的：观察和研究 iNOS 在高血糖加重脑缺血再灌注损伤中的表达及影响，为糖尿病／高血糖脑中风患者的预防和治疗提供新的靶点和思路。方法：建立动物模型，采用免疫组化技术检测 iNOS 因子的表达情况。结果：在正常血糖手术组再灌注1d 时 iNOS 阳性细胞数量增加，再灌注3d 时达到高峰，7d 后下降，高血糖组各亚型组表达趋势同于正常血糖组，但同时间段则高于正常血糖组。结论：缺血再灌注损伤引发机体氧化应激反应，在高血糖条件下，氧化应激反应比血糖正常情况下更加严重，脑缺血再灌注损伤也加重。
Abstract
Objective observed and studied of iNOS in hyperglycemia cerebral ischemia -reperfusion injury in rats and ef ect,thus providing a new target for diabetes /high blood sugar prevention and treatment of stroke patients and ideas.Methods SD rat as animal model.Immunohistochemical staining techniques to detect iNOS factors and the relationship between astrocytes in-jury.Results iNOS immunohistochemistry Results:In normoglycemic surgery group 1d after reperfusion surgical group when the number of iNOS -positive cel s increased,reaching a peak reper-fusion 3d,7d after fal down to the stable stage 14d,the hyperglycemia group each isoform expression group with normal glucose group trend,but at the same time is higher than normal blood sugar group.Conclusion Ischemic reperfusion injury caused by oxidative stress,oxidative stress is more severe than normoglycemic.
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