首页|Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii

Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
Mogrosides and steroid saponins are tetracyclic triterpenoids found in Siraitia grosvenorii.Squalene synthase (SQS) and cycloartenol synthase (CAS) are key enzymes in triterpenoid and steroid biosynthesis.In this study,full-length cDNAs of SgSQS and SgCAS were cloned by a rapid amplification of cDNA-ends with polymerase chain reaction (RACE-PCR) approach.The SgSQS cDNA has a 1254 bp open reading frame (ORF) encoding 417 amino acids,and the SgCAS cDNA contains a 2298 bp ORF encoding 765 amino acids.Bioinformatic analysis showed that the deduced SgSQS protein has two transmembrane regions in the C-terminal.Both SgSQS and SgCAS have significantly higher levels in fruits than in other tissues,suggesting that steroids and mogrosides are competitors for the same precursors in fruits.Combined in silico prediction and subcellular localization,experiments in tobacco indicated that SgSQS was probably in the cytoplasm or on the cytoskeleton,and SgCAS was likely located in the nucleus or cytosol.These results will provide a foundation for further study of SgSQS and SgCAS gene functions in S.grosvenorii,and may facilitate improvements in mogroside content in fruit by regulating gene expression.

Siraitia grosvenoriiTriterpenoidsSteroidsCloningExpressionSqualene synthaseCycloartenol synthaseSubcellular localization

Huan Zhao、Qi Tang、Changming Mo、Longhua Bai、Dongping Tu、Xiaojun Ma

展开 >

The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100194, China

Hunan Provincial Key laboratory of Crop Germplasm Innovation and Utilization and National Chinese Medicinal Herbs(Hunan)Technology Center, Hunan Agricultural University, Changsha 410128, China

Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, China

This work was supported by the National Nature Science Foundation of ChinaNational Key Technology Support ProgramGuangxi Natural Science FundationGuangxi Natural Science Fundation

813739142011BAI01B032013GXNSFDA0190212012GXNSFAA053043

2017

药学学报(英文版)

药学学报(英文版)

CSTPCDCSCD
ISSN：
年,卷(期)：2017.7(2)
  • 11
  • 30