首页|CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery

CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery

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The short release half-life of carbon monoxide(CO)is a major obstacle to the effective ther-apeutic use of carbon monoxide-releasing molecule-2(CORM-2).The potential of CORM-2-entrapped ultradeformable liposomes(CORM-2-UDLs)to enhance the release half-life of CO and alleviate skin inflammation was investigated in the present study.CORM-2-UDLs were prepared by using soy phospha-tidylcholine to form lipid bilayers and Tween 80 as an edge activator.The deformability of CORM-2-UDLs was measured and compared with that of conventional liposomes by passing formulations through a filter device at a constant pressure.The release profile of CO from CORM-2-UDLs was evaluated by myoglobin assay.In vitro and in vivo anti-inflammatory effects of CORM-2-UDLs were assessed in lipopolysaccharide-stimulated macrophages and TPA-induced ear edema model,respectively.The deformability of the optimized CORM-2-UDLs was 2.3 times higher than conventional liposomes.CORM-2-UDLs significantly prolonged the release half-life of CO from 30 s in a CORM-2 solution to 21.6 min.CORM-2-UDLs demonstrated in vitro anti-inflammatory activity by decreasing nitrite pro-duction and pro-inflammatory cytokine levels.Furthermore,CORM-2-UDLs successfully ameliorated skin inflammation by reducing ear edema,pathological scores,neutrophil accumulation,and inflamma-tory cytokines expression.The results demonstrate that CORM-2-UDLs could be used as promising ther-apeutics against acute skin inflammation.

Carbon monoxideCORM-2Anti-inflammatory effectUltradeformable liposomesSkin inflammationEar edema

Gwan-Yeong Lee、Alam Zeb、Eun-Hye Kim、Beomseon Suh、Young-Jun Shin、Donghyun Kim、Kyoung-Won Kim、Yeong-Hwan Choe、Ho-Ik Choi、Cheol-Ho Lee、Omer Salman Qureshi、In-Bo Han、Sun-Young Chang、Ok-Nam Bae、Jin-Ki Kim

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College of Pharmacy,Institute of Pharmaceutical Science and Technology,Hanyang University,Ansan 15588,Republic of Korea

Riphah Institute of Pharmaceutical Sciences,Riphah International University,Islamabad 44000,Pakistan

Department of Pharmacy,Forman Christian College,Lahore 54600,Pakistan

Department of Neurosurgery,CHA Bundang Medical Center,School of Medicine,CHA University,Seongnam 13496,Republic of Korea

College of Pharmacy,Ajou University,Suwon 16499,Republic of Korea

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Basic Science Research Program of the National Research Foundation of Korea(NRF)funded by the Ministry of Science and ICT

NRF-2017R1A2B4006458

2020

药学学报(英文版)

药学学报(英文版)

CSTPCDCSCDSCI
ISSN：
年,卷(期)：2020.10(12)
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