pH-Responsive polymer boosts cytosolic siRNA release for retinal neovascularization therapy

扫码查看

原文链接

万方数据
维普

外文摘要：Small interfering RNA(siRNA)has a promising future in the treatment of ocular diseases due to its high efficiency,specificity,and low toxicity in inhibiting the expression of target genes and pro-teins.However,due to the unique anatomical structure of the eye and various barriers,delivering nucleic acids to the retina remains a significant challenge.In this study,we rationally design PACD,an A-B-C type non-viral vector copolymer composed of a hydrophilic PEG block(A),a siRNA binding block(B)and a pH-responsive block(C).PACDs can self-assemble into nanosized polymeric micelles that compact siRNAs into polyplexes through simple mixing.By evaluating its pH-responsive activity,gene silencing efficiency in retinal cells,intraocular distribution,and anti-angiogenesis therapy in a mouse model of hypoxia-induced angiogenesis,we demonstrate the efficiency and safety of PACD in delivering siRNA in the retina.We are surprised to discover that,the PACD/siRNA polyplexes exhibit remarkable intracellular endosomal escape efficiency,excellent gene silencing,and inhibit retinal angiogenesis.Our study provides design guidance for developing efficient nonviral ocular nucleic acid delivery systems.

外文关键词：

siRNAOcular delivery systemBlock polymerEndosomal escapeRetinal neovascularizationBlood-retinal barrierVEGFASafety

作者：

Shuai Guo、Chunhui Li、Changrong Wang、Xiaowen Cao、Xinyue Liu、Xing-Jie Liang、Yuanyu Huang、Yuhua Weng

展开 >

作者单位：

School of Medical Technology,Advanced Research Institute of Multidisciplinary Science,School of Life Science,Key Laboratory of Molecular Medicine and Biotherapy,Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering,Beijing Institute of Technology,Beijing 100081,China

School of Pharmacy,Shandong New Drug Loading & Release Technology and Preparation Engineering Laboratory,Binzhou Medical University,Yantai 264003,China

School of Ophthalmology and Optometry,School of Biomedical Engineering,The Eye Hospital,Wenzhou Medical University,Wenzhou 325027,China

Chinese Academy of Sciences(CAS),Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology of China,Beijing 100190,China

展开 >

基金：

National Natural Science Foundation,ChinaNational Natural Science Foundation,ChinaNational Natural Science Foundation,ChinaNational Natural Science Foundation,ChinaNational Natural Science Foundation,ChinaNational Natural Science Foundation,ChinaNational Key Research & Development Program of ChinaNational Key Research & Development Program of ChinaNational Key Research & Development Program of ChinaFundamental Research Funds for the Central Universities,ChinaBeijing Nova Program(Interdisciplinary Cooperation Project)from Beijing Municipal Science & Technology Commission,China

项目编号：

3200100832171394319010533210115732101148822023382021YFA12010002021YFE01069002021YFC23024002022CX0101320220484207

出版年：

2024

DOI：

10.1016/j.apsb.2023.09.001

药学学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.14(2)

参考文献量27