Role of Iron Metabolism Disorder Regulated by GRSF1/GPX4 Axis in Cerebral Ischemia-reperfusion Injury in mice
Objective To investigate the role of iron metabolism disorder regulated by GRSF1/GPX4 axis in cerebral ischemia-reperfusion injury in mice.Methods A total of 18 clean grade male C57BL/6mice were randomly divided into 3groups:sham operation group,cerebral ischemia-reperfusion group,cerebral ischemia-reperfusion+GRSF1 overexpression group,with 6mice in each group.The model of cerebral ischemia-reperfusion was established by thread occlusion in cerebral ischemia-reperfusion group,and GRSF1 overexpressed lentivirus was injected in cerebral ischemia-reperfusion+GRSF1 overexpression group 7days before modeling.After 24h of reperfusion,the neurological function score was evaluated,apoptosis rate of brain tissue was detected by TUNEL method,and the protein expression levels of GRSF1,GPX4,IRP2,TfR1 and ferritin were detected by Western blotting method.Results Compared with sham operation group,the neurological function score,apoptosis rate were increased as well as the expression levels of IRP2,TfR1 and ferritin in cerebral ischemia-reperfusion group,while GRSF1,GPX4 expression levels were decreased(P＜0.05).Compared with cerebral is-chemia-reperfusion group,the neurological function score,apoptosis rate,the expression levels of IRP2,TfRl and ferritin were de-creased in cerebral ischemia-reperfusion+GRSF1 overexpression group,while GRSF1 and GPX4 expression levels were increased(P＜0.05).Conclusion Overexpression of GRSF1 attenuates cerebral ischemia-reperfusion injury in mice by up-regulating GPX4 to in-hibit iron metabolism disorder.
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