Marein Increases Sensitivity of Ovarian Cancer Cells to DNA Damage Drugs by Targeting p38α
Objective To investigate the efficacy and mechanism of marein combined with DNA damage drugs by inhibiting homolo-gous recombination repair.Methods CCK-8method,clonal colony formation assay and organoid drug sensitivity test were used to detect the sensitization of marein combined with DNA damage drugs.Western blot,comet assay and homologous recombination repair reporter gene were used to detect the DNA damage and the mechanism of inhibiting the homologous recombination repair.The binding of marein to p38α was confirmed by cell heat transfer assay and target stability technique of drug affinity reaction.Results Marein increased the sus-ceptibility of cancer cells and organoids to DNA damage drugs by binding to p38α.By inhibiting p-p38α,marein increased DNA damage of cancer cells,and inhibited the homologous recombination repair of cancer cells.Conclusion Marein enhances the sensitivity of cancer cells to DNA damage drugs by inhibiting homologous recombination repair,which provides a new strategy for clinical use of ovarian cancer.
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