Ameliorative Effect of Delayed Mild Hypothermia on White Matter Injury in Neonatal Rats
Objective To investigate the ameliorative effect of delayed mild hypothermia on white matter injury induced by oxygen-glucose deprivation and restoration in neonatal rats and its possible mechanism.Methods Forty neonatal rats were randomly divided into four groups for delayed mild hypothermia,10 rats in each group.The expression of myelin basic protein(MBP)and the number of oligo-dendrocyte progenitor cells(O4)in brain sections of each group were analyzed by immunofluorescence.The gene expression in M1 and M2 microglia was detected by real-time quantitative polymerase chain reaction(RT-qPCR).Results The fluorescence intensity of MBP protein in the mild low temperature 32℃ delay for 24h,48h and 72h groups were higher than those in the control group,and the flu-orescence intensity increased with the increase of the delay time.The number of O4 cells was higher than that of control group(F=314.907,P＜0.001),and the increase of cell number was significantly positively correlated with delay time(r=0.968,P＜0.001).The expression of CD32 and iNOS in M1 microglia was lower than that in control group(F were 41.451,92.912,all P＜0.001).The ex-pression of CD32 and iNOS was negatively correlated with the delay time(F were-0.868,-0.916,all P＜0.001).The expression of CD206 and IL-10 in M2 microglia was higher than that in control group(F were 79.699,63.839,all P＜0.001).The expressions of CD206 and IL-10 were significantly negatively correlated with the delay time(F were 0.862,0.910,all P＜0.001).Conclusion De-layed mild hypothermia can effectively ameliorate the damage of white matter injury induced by oxygen-glucose deprivation and restoration in neonatal rats,its mechanism may be related to increase O4 quantity and promote microglia M1 to M2 polarization.
White matter injuryNeonatal ratMild hypothermiaOligodendrocyte precursor cellMyelin Basic ProteinMicroglia
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NSTL
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