Analysis of m6A Modification-related Long Non-coding RNA on Prognosis and Immunotherapy of Clear Cell Renal Cell Carcinoma
Objective To investigate the prognostic and immunotherapy characteristics of N6-methyladenine(m6A)-related long non-coding RNA(lncRNA)in renal clear cell carcinoma(ccRCC).Methods Transcriptome data and clinical characteristic data of 532 ccRCC tissues and 72 normal kidney tissues were downloaded from the TCGA database,and lncRNAs related to prognosis and m6A were screened by co-expression and univariate COX regression analysis.Cluster analysis was performed based on the prognosis and the expression of m6A-related lncRNA,the correlation between the two clusters and the expression of immune checkpoints programmed death-ligand 1(PD-L1)and cytotoxic T lymphocyte-associated antigen-4(CTLA-4)was analyzed.The LASSO COX regression risk prognosis model was constructed to verify its clinical application value,and the ESTIMATE method was used to compare the differ-ences in the tumor microenvironment of ccRCC,and the immunophenoscore(IPS)predicted the sensitivity of ccRCC to immune check-point inhibitor(ICI).Results Twenty-nine lncRNAs associated with prognosis and m6A were screened by co-expression and univari-ate COX regression analysis,most of which were up-regulated in ccRCC tissues,while COL18A1-AS1,AC007066.2 and AC018752.1 were down-regulated.The 532 ccRCC tissue samples were divided into cluster1group and cluster2group by cluster analysis.The clus-ter2group had worse prognosis and higher expression of PD-L1 and CTLA4.Eight lncRNAs related to prognosis and m6A were identified by LASSO COX regression algorithm to establish a ccRCC risk prognostic model,which has good predictive value in different clinical fea-tures.Patients were divided into high-risk group and low-risk group based on risk scores calculated by the risk prognostic model.Pa-tients in the high-risk group had a worse prognosis,more immune cell components,and were more sensitive to ICI treatment.Conclu-sion m6A-related lncRNA is of great significance for the prognosis prediction and immunotherapy guidance in ccRCC patients.
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