lncRNA MALAT1 Regulates Wnt/β-catenin by Sponge Adsorption of MiRNA-141-3p to Promote the Growth and Metastasis of Ovarian Cancer
Objective To study the role and regulatory mechanism of long non-coding RNA MALAT1 in ovarian cancer.Methods Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of MALAT1 in human normal ovarian epithelial cell line IOSE80 and human ovarian cancer cell line SKOV3,OVCA429 and HO-8910PM.SKOV3 and HO-8910PM cells were selected for the next study.The expression of MALAT1 in SKOV3 and HO-8910PM cells were interfered with siRNA,the cell pro-liferation was detectd by CCK-8 method,the cell migration and invasion were detectd by scratche and Transwell assay respectively,and the expression of epithelial-mesenchymal transformation(EMT)-related indicators E-cadherin and N-cadherin were detected by Western blot.The targeting relationship between MALAT1 and miRNA-141-3p was analyzed by bioinformatics,and was verified by du-al luciferase reporter.MALAT1 knockdown and miRNA-141-3p inhibitor were treated the SKOV3 and HO-8910PM cells at the same time,then the cell proliferation,invasion and migration were detected,Western blot was used to detect the expression of Wnt/β-catenin signaling pathway related proteins.Results Compared with human normal ovarian epithelial cell line IOSE80,the expression of MALAT1 in ovarian cancer cell line was significantly up-regulated(P<0.05).When the expression of MALAT1 in SKOV3 and HO-8910PM was down-regulated,the cell proliferation,invasion,migration and EMT were inhibited(P<0.05).At the same time,the expression of miRNA-141-3p was up-regulated(P<0.05),and the results of dual luciferase reporter proved that there was a targeting relation-ship between MALAT1 and miRNA-141-3p.However,the inhibitory effect of MALAT1down-regulation in the cells can be reversed by miRNA-141-3p inhibitor(P<0.05).Further studies had found that the MALAT1/miRNA-141-3p axis may influence the pro-gression of ovarian cancer by regulating the Wnt/β-catenin signaling pathway.Conclusion MALAT1may influence the occurrence and development of ovarian cancer by sponge adsorption of miRNA-141-3 via regulating Wnt/β-catenin.
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