NEDD4-1 is Involved in the Occurrence and Development of Pancreatic Cancer by Regulating the JAK2/STAT3Pathway
Objective To investigate the role and mechanism of neural precursor cell expressed,developmentally down-regulated 4-1(NEDD4-1)in the occurrence and development of pancreatic cancer tissue and cell.Methods The expression of NEDD4-1 in pancreatic ductal adenocarcinoma and its adjacent tissues was detected by immunohistochemistry.The expression of NEDD4-1 in pancre-atic cancer cells and normal pancreatic ductal epithelial cells was detected by Western blot.The expression of NEDD4-1 in human pan-creatic carcinoma PANC-1 cells was silenced by siRNA,and the changes of growth,proliferation,apoptosis and migration activity were detected,and the changes of NEDD4-1,p-JAK2 and p-STAT3 protein expression in the cells before and after silencing were investi-gated.Results The expression level of NEDD4-1 in pancreatic ductal adenocarcinoma was significantly higher than that in adjacent tis-sues(P<0.01).The expression level of NEDD4-1 protein in pancreatic cancer cells was also significantly higher than that in normal pancreatic ductal epithelial cells(P<0.01).The expression level of NEDD4-1 protein in siNEDD4-1 group was only 41.71%±4.58%of that in siNC group at 36h after transfection,and the difference between two groups was statistically significant(P<0.01).Compared with siNC group,the growth and proliferation activity of PANC-1 cells in siNEDD4-1 group was decreased,while the apopto-sis rate was increased,and the protein expressions of NEDD4-1,p-JAK2 and p-STAT3 were significantly down-regulated(P<0.05).Conclusion The expression of NEDD4-1 is increased in pancreatic cancer tissues and cells.Silencing of NEDD4-1 may in-hibit the proliferation and migration activity of pancreatic cancer cells and induce cell apoptosis by regulating JAK2/STAT3 pathway.NEDD4-1may be a new target for the treatment of pancreatic cancer.
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