目的 探讨LINC00638在结肠癌中的表达及其临床意义,并进一步探究LINC00638对结肠癌细胞生物学功能的影响.方法 通过癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库初步比较LINC00638在正常结肠组织和结肠癌组织中的表达差异,探究其与患者临床病理特征的关系.通过Kaplan-Meier生存分析进一步探究LINC00638表达对结肠癌患者预后的影响.通过GEPIA数据库和高通量基因表达数据库(Gene Expression Omnibus,GEO)进一步验证LINC00638表达对结肠癌患者预后的影响.采用单因素和多因素COX回归分析探究LINC00638、临床病理相关因素对结肠癌患者预后的影响.应用小干扰RNA(siRNA)沉默HCT116细胞和DLD1细胞中LINC00638的表达,通过CCK-8实验、集落形成实验、划痕实验、Transwell实验探究LINC00638对结肠癌细胞增殖、迁移和侵袭能力的影响.结果 LINC00638在结肠癌组织中表达水平明显升高,并且其表达量与结肠癌患者的淋巴结转移和TNM分期密切相关.Kaplan-Meier生存曲线分析结果显示,LINC00638表达水平越高,结肠癌患者预后越差(P＜0.05).COX回归分析结果显示,LINC00638、年龄和TNM分期可以作为结肠癌患者的独立预后因素.细胞功能实验结果表明,与对照组比较,敲低组细胞的增殖、迁移和侵袭能力显著降低(P＜0.05).结论 LINC00638在结肠癌中高表达,并且其表达水平越高,结肠癌患者预后越差.敲低LINC00638后可以抑制结肠癌细胞的增殖、迁移和侵袭能力.
Expression of LINC00638 in Colon Cancer and its Study on the Function of Colon Cancer Cells
Objective To investigate the expression and clinical significance of LINC00638 in colon cancer,and further to explore the impact of LINC00638 on the biological function of colon cancer cells.Methods The cancer genome atlas(TCGA)database was used to preliminarily compare the expression differences of LINC00638 in normal colon tissue and colon cancer tissue,and to explore the rela-tionship between LINC00638 and clinical pathological characteristics of patients.Kaplan-Meier survival analysis was used to further in-vestigate the effect of LINC00638 expression on the prognosis of colon cancer patients.The GEPIA database and the Gene Expression Om-nibus(GEO)database were used to further validate the effect of LINC00638 expression on the prognosis of colon cancer patients.Univari-ate and multivariate COX regression analysis were used to explore the effect of LINC00638,clinical and pathological related factors on the prognosis of colon cancer patients.Small interfering RNA(siRNA)was used to silence the expression of LINC00638 in HCT116 cells and DLD1 cells,and the effect of LINC00638 on the proliferation,migration and invasion of colon cancer cell was explored through CCK-8 experiment,colony formation experiment,scratch experiment and Transwell experiment.Results The expression level of LINC00638 was significantly increased in colon cancer tissue,and its expression level was closely related to lymph node metastasis and TNM staging in co-lon cancer patients.The results of Kaplan-Meier survival curve analysis showed that the higher the expression level of LINC00638,the worse the prognosis of colon cancer patients(P＜0.05).The results of COX regression analysis showed that LINC00638,age,and TNM staging could be independent prognostic factors for colon cancer patients.The results of cell function experiments showed that compared with the control group,the proliferation,migration,and invasion ability of cells in knockdown group were significantly decreased(P＜0.05).Conclusion LINC00638 is highly expressed in colon cancer,and the higher its expression level,the worse the prognosis of colon cancer patients.After knocking down LINC00638,it can inhibit the proliferation,migration and invasion ability of colon cancer cells.
万方数据
维普
