TREM1在食管癌中的表达意义及促进食管癌转移的相关机制研究
Expression Significance of TREM1 in Esophageal Cancer and the Related Mechanisms of Promoting Esophageal Cancer Metastasis
孙闻 1汤翔 1余云鹏 1苏玉婷 1陈德玉1
作者信息
摘要
目的 分析髓样细胞触发受体1(triggering receptors expressed on myeloid cells-1,TREM1)在食管癌中的表达情况以及与患者总体生存率的关系,并探究其对食管癌KYSE30细胞迁移能力的影响.方法 通过生物信息学及临床标本分析TREM1在肿瘤组织中的表达情况及与患者预后的关系;选择KYSE30细胞,将细胞分为阴性对照(NC)组、阴性对照+脂多糖(NC+LPS)组、干扰TREM1组、干扰TREM1+脂多糖组.采用Transwell实验比较不同组别细胞增殖和迁移能力的变化;采用Western blot法检测不同组别间上皮间充质转化(epithelial-mesenchymal transition,EMT)相关蛋白的变化;采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)比较不同组别细胞白细胞介素-1 β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-8(interleukin-8,IL-8)的变化;过表达TREM1的同时干扰IL-6,比较此时EMT相关蛋白的变化.结果 TREM1在食管癌组织和患者血清中表达丰度较高,并与患者的淋巴结转移和总体生存率有关;TREM1可以促进KYSE30细胞的侵袭和转移,相关机制可能是通过促进IL-6的表达进而启动食管癌细胞EMT.结论 TREM1在食管癌组织中高表达并通过上调IL-6促进肿瘤EMT从而有利于食管癌的侵袭和转移,导致患者预后不良.
Abstract
Objective To analyze the expression of triggering receptors expressed on myeloid cells-1(TREM1)in esophageal cancer and its relationship with the overall survival rate of patients,and to explore its effect on the migration ability of KYSE30 cell in e-sophageal cancer.Methods The expression of TREM1 in tumor tissue and its relationship with patient prognosis were analyzed by bioin-formatics and clinical specimens.KYSE30 cells were selected and divided into negative control(NC)group,negative control+lipopo-lysaccharide(NC+LPS)group,interference TREM1 group,interference TREM1+lipopolysaccharide group.Compare the changes in cell proliferation and migration ability of different groups using Transwell method;Western blot was used to detect changes in EMT-relat-ed proteins among different groups;real-time quantitative polymerase chain reaction(RT-qPCR)was used to compare interleukin-1β(IL-1β),interleukin-6(IL-6)and interleukin-8(IL-8)of different groups of cells;overexpression of TREM1 interfered with IL-6,and the changes in EMT-related proteins were compared at this time.Results TREM1 was highly expressed in esophageal cancer tissue and serum,and was associated with lymph node metastasis and overall survival rate in patients;TREM1 can promote the in-vasion and metastasis of KYSE30 cells,and the related mechanism may be by promoting the expression of IL-6,thereby initiating EMT in esophageal cancer cells.Conclusion TREM1 is highly expressed in esophageal cancer tissue,and promotes tumor EMT through up-regulation of IL-6,which is beneficial for the invasion and metastasis of esophageal cancer,leading to poor prognosis in patients.
关键词
TREM1/食管癌/白细胞介素-6/上皮间充质转化/侵袭和转移Key words
TREM1/Esophageal cancer/IL-6/EMT/Invasion and metastasis引用本文复制引用
基金项目
国家自然科学基金资助项目(面上项目)(81572956)
江苏省社会发展基金资助项目(BE2017696)
江苏省医学创新团队项目(CXTDC2016009)
出版年
2024
NETL
NSTL
万方数据