目的 检测基质Gla蛋白(matrix Gla protein,MGP)、信号转导及转录激活因子3(signal transduction and activator of transcription 3,STAT3)、β-联蛋白(β-catenin)及基质金属蛋白酶 2(matrix metalloproteinase 2,MMP2)在胃腺癌及萎缩性胃炎中的表达,并探讨四者的相关性.方法 通过Western blot法及免疫组化实验检测MGP、STAT3、β-catenin、MMP2蛋白的相对表达水平,并探讨四者与胃腺癌及萎缩性胃炎的相关性.结果 Western blot法结果显示,萎缩性胃炎组MGP、STAT3、β-catenin、MMP2蛋白相对表达水平均高于非萎缩性胃炎组,差异均有统计学意义(P＜0.0125);胃腺癌组MGP、STAT3、β-catenin、MMP2蛋白相对表达水平均高于非萎缩性胃炎组及萎缩性胃炎组,差异均有统计学意义(P＜0.05).免疫组化实验结果显示,萎缩性胃炎组MGP、STAT3、β-catenin、MMP2阳性率均高于非萎缩性胃炎组;胃腺癌组MGP、STAT3、β-catenin、MMP2阳性率均高于非萎缩性胃炎组及萎缩性胃炎组;除STAT3在非萎缩性胃炎组及萎缩性胃炎组差异无统计学意义外,其余组间差异均有统计学意义(P＜0.0125);在萎缩性胃炎及胃腺癌组织中,MGP、STAT3、β-catenin、MMP2的表达均呈正相关(P＜0.05).结论 MGP在胃腺癌及萎缩性胃炎中可能通过JAK2/STAT3信号通路及Wnt/β-catenin信号通路激活下游MMP2促进其发生和发展.
Expression and Correlation of MGP and Related Factors in Gastric Adenocarcinoma and Atrophic Gastritis
Objective To detect the expression of matrix Gla protein(MGP),signal transduction and activator of transcription 3(STAT3),β-catenin and matrix metalloproteinase 2(MMP2)in gastric adenocarcinoma and atrophic gastritis,and investigate the cor-relation of them.Methods The relative expression levels of MGP,STAT3,β-catenin and MMP2 proteins were detected by Western blot experiment and immunohistochemical experiment,and the correlation between the four proteins and gastric adenocarcinoma and a-trophic gastritis was investigated.Results The results of Western blot showed that the relative expression levels of MGP,STAT3,β-catenin and MMP2 proteins in atrophic gastritis group were higher than those in non-atrophic gastritis group,and the differences were statistically significant(P＜0.05).The relative expression levels of MGP,STAT3,β-catenin and MMP2 proteins in gastric adenocarci-noma group were higher than those in non-atrophic gastritis group and atrophic gastritis group,and the differences were statistically sig-nificant(P＜0.05).The results of immunohistochemical experiment showed that the positive rates of MGP,STAT3,β-catenin and MMP2 in atrophic gastritis group were higher than those in non-atrophic gastritis group.The positive rates of MGP,STAT3,β-catenin and MMP2 in gastric adenocarcinoma group were higher than those in non-atrophic gastritis group and atrophic gastritis group;except that STAT3 was not statistically significant in non-atrophic gastritis group and atrophic gastritis group,and the differences among other groups were statistically significant(P＜0.0125).In atrophic gastritis and gastric adenocarcinoma,the expressions of MGP,STAT3,β-catenin and MMP2 were positively correlated(P＜0.05).Conclusion MGP may activate downstream MMP2 through JAK2/STAT3 signaling pathway and Wnt/β-catenin signaling pathway to promote the occurrence and development of gastric adenocarcinoma and a-trophic gastritis.
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