Correlation of CST4 and VEGF-A Expression with Pathological Features and Prognosis in Esophageal Squamous Cell Carcinoma
Objective To investigate the correlation between the expression of cysteine protease inhibitor S(CST4)and vascular endothelial growth factor-A(VEGF-A)in esophageal squamous cell carcinoma(ESCC)and clinical pathological parameters.Methods Immunohistochemistry was used to detect the expression of CST4 and VEGF-A in the tissues of 50 patients with ESCC,and the correla-tion between them and pathological parameters was analyzed.The enrichment and correlation of them were confirmed by combining the re-sults of Enrichr and GEO databases.Results Compared with adjacent tissues,the positive expression rates of CST4 and VEGF-A in ESCC was significantly increased(P＜0.05).There were significant differences in the positive expression rate of CST4 in ESCC with dif-ferent lymph node metastasis,depth of invasion,and neurovascular invasion(P＜0.05).There were significant differences in the positive expression rate of VEGF-A in ESCC with different lymph node metastasis,depth of invasion,neurovascular invasion,and pathological stage(P＜0.05).Kaplan-Meier survival analysis indicated that postoperative survival rate of patients was related to the expression of CST4 and VEGF-A,depth of invasion,TNM stage and lymph node metastasis(P＜0.05).Spearman correlation analysis showed a pos-itive correlation between the expression of CST4 and VEGF-A in ESCC tissues(r=0.601,P＜0.05).COX regression analysis showed that VEGF-A expression level,CST4 expression level,lymph node metastasis,depth of invasion and pathological stage were risk factors for survival(P＜0.05).Enrichment analysis showed that CST4 and VEGF-A play a synergistic role in cell migration and invasion.Conclusion CST4 and VEGF-A are higher expressed in ESCC tissues than normal tissues.They are involved in the development and metastasis of ESCC,and they are expected to be important indicators for the treatment and prognosis of ESCC.
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