Relationship between NLRP3 Inflammasome and its Mediated Pyroptosis and Contrast-induced Acute Kidney Injury after Acute Myocardial Infarction
Objective To investigate the relationship between NLRP3 inflammasome and its mediated pyroptosis and contrast-in-duced acute kidney injury(CI-AKI)after acute myocardial infarction.Methods Ten CI-AKI rat models after acute myocardial in-farction were constructed as the CI-AKI group,and 10healthy rats were used as the control group.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to detect the mRNA and protein expression levels of NLRP3 inflam-masome activation markers NLRP3 and caspase-1 in the two groups to clarify the relationship between NLRP3 and CI-AKI.The mor-phological changes of renal tissue in the two groups were observed by transmission electron microscopy,and the levels of interleukin-1 β(IL-1β)and interleukin-18(IL-18)were detected by enzyme-linked immunosorbent assay(ELISA)to clarify the relationship be-tween pyroptosis and CI-AKI.Rat renal tubular cells were cultured to construct NLRP3 overexpression,silencing and blank control groups to clarify the relationship between NLRP3 and pyroptosis.Results The results of RT-qPCR and Western blot showed that the expression of NLRP3mRNA and protein in CI-AKI group was higher than that in control group(P＜0.05).Transmission electron mi-croscopy can clearly see that the mitochondrial structure of the CI-AKI group was destroyed,the mitochondria were vacuolated,and the mitochondrial cristae were significantly reduced and unclear;in addition,the levels of pyroptosis-related inflammatory indicators IL-1 βand IL-18 in the CI-AKI group were higher than those in the control group.There were significant differences in pyroptosis-related in-flammatory indicators IL-1 β and IL-18 between rat renal tubular NLRP3 overexpression,silencing and blank control groups(P＜0.05).Conclusion The expression level of NLRP3 increased in CI-AKI after acute myocardial infarction,which induced pyroptosis;the higher the level of NLRP3,the more significant the cell pyroptosis.NLRP3 is expected to become a new biological marker of CI-AKI,providing a new reference for the diagnosis,treatment and prognosis evaluation of CI-AKI.
NETL
NSTL
万方数据
