目的 探讨S100A16在子宫内膜腺癌中的表达及其对子宫内膜腺癌细胞增殖、迁移和侵袭能力的影响.方法 应用免疫组化法检测S100A16在子宫内膜腺癌组和正常对照组中的表达差异.根据表达情况不同,将所有子宫内膜腺癌患者分为S100A16高表达组和低表达组,然后分析两组间临床病理特征的差异.将S100A16慢病毒转染至人子宫内膜腺癌细胞系Ishika-wa 和HEC-1A细胞,CCK-8法、划痕实验和Transwell实验分别检测S100A16-shNC组和S100A16-shRNA组间增殖、迁移及侵袭能力的不同,W estern blot法检测上皮间质转化(epithelial mesenchymal transformation,EMT)通路相关蛋白的表达.结果 S100A16在子宫内膜腺癌组中的表达显著高于正常对照组(P＜0.01).S100A16的高表达与子宫内膜腺癌患者病理分级更差有关(P＜0.05),与年龄、国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)分期和淋巴结转移方面无关(P＞0.05).CCK-8实验检测结果显示,敲低S100A16蛋白使子宫内膜腺癌细胞增殖能力减低(P＜0.01).划痕实验和Tran-swell实验显示,S100A16-shNC组的子宫内膜腺癌细胞迁移及侵袭能力高于S100A16-shRNA组(P＜0.05).Western blot法检测结果显示,与S100A16-shNC组比较,S100A16-shRNA组的子宫内膜腺癌细胞E-钙黏蛋白(E-cadherin)表达上调,N-钙黏蛋白(N-cadherin)和波形蛋白(Vimentin)表达下调(P＜0.05).结论 S100A16在子宫内膜腺癌组织中高表达,并通过诱导EMT促进子宫内膜腺癌细胞的恶性生物学行为.
Exploring the Role of S100A16 in Endometrial Adenocarcinoma Tissues and Cells
Objective To investigate the expression of S100A16 in endometrial adenocarcinoma and its effect on the proliferation,migration and invasion ability of endometrial adenocarcinoma cells.Methods Immunohistochemistry was applied to detect the differences in the expression of S100A16 in endometrial adenocarcinoma and normal controls.According to the expression status,all endometrial ade-nocarcinoma patients were divided into S100A16 high-expression group and low-expression group,and then the differences in clinico-pathological characteristics of endometrial adenocarcinoma patients between the two groups were analyzed.S100A16 lentivirus was trans-fected into human endometrial adenocarcinoma cell lines Ishikawa and HEC-1A cells.CCK-8,Scratch assay and Transwell assay were used to detect the differences in proliferation,migration and invasive ability between the S100A16-shNC group and the S100A16-shR-NA group,respectively.Western blot was performed to detect the expression of proteins related to the epithelial mesenchymal transforma-tion(EMT)pathway protein expression.Results The expression of S100A16 in endometrial adenocarcinoma tissues was significantly higher than that in normal controls(P＜0.01).High expression of S100A16 was associated with worse pathological grading in patients with endometrial adenocarcinoma(P＜0.05),independent of age,International Federation of Gynecology and Obstetrics(FIGO)stage and lymph node metastasis aspects(P＞0.05).The results of CCK-8 assay showed that knockdown of S100A16 protein reduced the pro-liferation ability of endometrial adenocarcinoma cells(P＜0.01).Scratch assay and Transwell assay showed that the migration and inva-sion ability of endometrial adenocarcinoma cells in the S100A16-shNC group was higher than that in the S100A16-shRNA group(P＜0.05).Western blot revealed that E-cadherin expression was up-regulated,N-cadherin and Vimentin expression was down-regula-ted in endometrial adenocarcinoma cells in the S100A16-shRNA group compared with the S100A16-shNC group(P＜0.05).Conclusion S100A16 is highly expressed in endometrial adenocarcinoma tissues and promotes the malignant biological behaviour of en-dometrial adenocarcinoma cells by inducing EMT.
NETL
NSTL
万方数据
