miR-198对肝癌细胞株增殖和迁移的影响
Effects of MiR-198 on Proliferation and Migration of Hepatocellular Carcinoma Cell Lines
王磊 1韩全乡 2李灵招 3徐文宁 2李醒亚4
作者信息
- 1. 450003 郑州大学附属郑州中心医院放疗科;450011 郑州,河南省中医院(河南中医药大学第二附属医院)
- 2. 450003 郑州大学附属郑州中心医院放疗科
- 3. 450003 郑州大学附属郑州中心医院检验科
- 4. 450003 郑州大学第一附属医院肿瘤科
- 折叠
摘要
目的 探讨miR-198对肝癌细胞株增殖和迁移的影响.方法 采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)法检测miR-198在肝癌的不同细胞株中的表达量,然后运用细胞实验及动物实验进行miR-198对Huh7细胞株增殖、迁移及成瘤能力的研究.结果 RT-qPCR检测结果显示,与Chang liver和LO2正常肝细胞株比较,HepG2、Huh7、Hep3B和MHCC97H肝癌细胞株中的miR-198表达量降低,其中在Huh7细胞中尤为明显;流式细胞术检测结果显示,miR-198能够促进Huh7细胞凋亡;CCK-8及软琼脂克隆形成实验结果显示,与对照组比较,Huh7肝癌细胞株在转染miR-198模拟物后其增殖能力显著降低;划痕实验及Transwell实验检测结果显示,miR-198能够抑制Huh7肝癌细胞株的迁移能力;裸鼠成瘤实验结果显示,转染miR-198模拟物的Huh7肝癌细胞株的成瘤能力受到明显抑制.结论 miR-198在HepG2、Huh7、Hep3B和MHCC97H肝癌细胞株中的表达量明显降低,且在Huh7肝癌细胞株中最低;miR-198能够抑制Huh7肝癌细胞株增殖及迁移能力,降低其成瘤功能.
Abstract
Objective To investigate the effects of miR-198 on proliferation and migration of hepatocellular carcinoma cell lines.Methods RT-qPCR was used to detect the expression of miR-198 in different hepatocellular carcinoma cell lines,and the prolifera-tion,migration and tumorigenic ability of miR-198 on Huh7 cell lines were identified by in cell experiments and in animal experiments.Results RT-qPCR test results showed that compared with Chang liver and LO2 normal liver cell lines,the expression levels of miR-198 in HepG2,Huh7,Hep3B and MHCC97H hepatocellular carcinoma cell lines were decreased,especially in Huh7 cells.Flow cytome-try was used to confirm that miR-198 promoted the apoptosis of Huh7 cells.CCK-8 and soft agar clone formation experiment showed that compared with the control group,the proliferation of Huh7 hepatocellular carcinoma cell line after transfection with miR-198mimics was significantly reduced.The results of scratch test and Transwell test showed that miR-198 could inhibit the migration ability of Huh7 hepatocellular carcinoma cell line.The tumor formation experiment in nude mice proved that the tumor-forming ability of Huh7 hepato-cellular carcinoma cell line transfected with miR-198 was obviously inhibited.Conclusion The expression level of miR-198 in HepG2,Huh7,Hep3B and MHCC97H hepatocellular carcinoma cell lines was significantly decreased,and the lowest expression was found in Huh7 hepatocellular carcinoma cell line.MiR-198 can inhibit the proliferation and migration ability of Huh7 hepatocellular car-cinoma cell line,and reduce its tumor-forming ability.
关键词
miR-198/肝癌细胞株/增殖/迁移Key words
MiR-198/Hepatocellular carcinoma cell lines/Proliferation/Migration引用本文复制引用
出版年
2024
NETL
NSTL
万方数据