Antioxidant Effect of Hydrogen on Hypoxic-ischemic Brain Injury in Neonatal Rats
Objective To detect the biomarkers closely related to oxidative stress and antioxidant defense mechanisms[superoxide dismutase(SOD),glutathione peroxidase(GSH-PX)and malondialdehyde(MDA)]in neonatal rats Changes in different treatment regimens for hypoxic-ischemic brain injury in rats,and explore the antioxidant effect of hydrogen.Methods HIE animal model was made from 7-day-old newborn SD rats,and then randomly divided into sham operation group,control group,test group 1(saturated hydrogen saline group),test group 2(mild hypothermia group),and test group 3(combination group).Experimental group 1 was intrap-eritoneally injected with saturated hydrogen saline after modeling;experimental group 2 was treated with mild hypothermia after modeling;experimental group 3 was treated with mild hypothermia combined with injection of saturated hydrogen saline after modeling.The control group only had modeling,and the sham operation group did not.For special treatment,10 rats from each group were decapitated 1day,3days,and 7days after modeling,and brain tissue was collected to detect the concentrations of SOD,GSH-PX,and MDA.Results In the control group,the expression levels of SOD and GSH-PX decreased on the 1st day after modeling,became more obvious on the 3rd day,and increased somewhat on the 7th day.The expression of MDA increased,became more obvious on the 3rd day,and decreased somewhat on the 7th day,which was similar to that of the sham operation.The comparisons between groups at each stage were statistically significant(P＜0.05),and the expression levels of SOD and GSH-PX in experimental groups 1 and 2 at the three stages after modeling were higher than those in the control group and lower than those in experimental groups 3 and 2.In the sham operation group,the MDA expression levels were lower than those in the control group and higher than those in the experimental group 3 and the sham operation group,with statistical significance(P＜0.05).There was no statistical significance in the comparison between experimental group 1 and experimental group 2 at each stage(P＞0.05).Conclusion Saturated hydrogen saline treatment can protect neonatal rats from hypoxic-ischemic brain injury to a certain extent by reducing the level of oxidative stress in the body,while the protective effect of mild hypothermia combined with hydrogen treatment on HIE is time-sensitive.
万方数据
维普
