Objective To investigate the value of follow-up analysis of indeterminate breast calcifications by mammography in the differential diagnosis of benign and malignant breast lesions.Methods A total of 56 cases were retrospectively analyzed from April 2020 to January 2023,which were divided into benign and malignant groups,including 19 cases in the malignant group and 37 cases in the benign group,after the discovery of indeterminate calcifications by mammography and at least 1 follow-up mammogram at Guangdong Second Hospital of Traditional Chinese Medicine,which were confirmed by surgical pathology.The two groups of patients were analyzed by the following four indicators.(1)Whether the number of indeterminate calcifications increased between the two follow-ups.(2)The number of increased calcifications between the two follow-ups.(3)Whether the morphology of the indeterminate calcifications changed between the two follow-ups.(4)Whether there was a new increase in glandular density.Results The incidence of increased calcification in the malignant group was 78.95%,higher than 43.24%in the benign group;the increase in calcification in the malignant group was greater than that in the benign group;the incidence of calcification morphological changes in the malignant group was 78.95%,which was higher than 13.51%in the benign group;the incidence of increased local glandular density in the malignant group was 68.42%,which was higher than the benign group's 18.92%,and the differences were statistically significant(P<0.05).Conclusion Follow-up and analysis of indeterminate breast calcifications by mammography is of high value in the differential diagnosis of benign and malignant breast disease.