Objective To investigate the effect of ecological factors on the accumulation of these two components,and to provide the scientific basis for improving the quality standard of the A rtemisia leaves.Methods The content of eupatilin and jaceosidinin 9 batches of leaves from different producing areas were determined by high performance liquid chromatography,the chromatographic column was Kromasil 100-5 C18 (4.6 mm×150 mm,5 μm).Mobile phase was acetonitrile-sodium dihydrogen phosphate buffer (70∶30,V/V) with an isocratic elution.The data of four ecological factors,including altitude,annual average temperature,annual average precipitation and soil organic carbon content,were extracted by ARCGIS software combined with the use of various spatial data.Results The 9 batches of Artemsiaargyi sample sofeupatilin and jaceosidin two components respectively in 0.02-0.5 mg/mL and 0.04-0.1 g/mL and peak area showed a good linear relationship.The content of active components in each sample was different from that of the sample producing area.The contents of eupatilin and jaceosidin were between 0.110％-0.276％ and 0.059％-0.072％,respectively.The content change of eupatilin was obvious,while jaceosidin had a small change.The highest content of the two components were Hubei Qichun samples.Conclusion The contents of the two components of eupatilin and jaceosidin are affected by the unique ecological factors,manifested as real estate areas is significantly higher than that of non real estate areas,and the content of eupatilinis closely related to the quality of herbs,can be used for the quality evaluation and control of A rtemisia herbs.
NETL
NSTL
维普
万方数据
