Isolation,purification and antioxidant activity of isolithospermic acid
To optimize the separation and purification technology of isolithospermic acid from extract of salvia polyphenolic acids,and study the antioxidant activity in vitro.The high purity isolithospermic acid was isolated by MCI Gel column,LH-20 Gel column,dynamic axial pressure preparation liquid chromatography,and the result was compared with those of silica gel column chromatography.The antioxidant activity of isolithospermic acid in vitro was analyzed by OH scavenging test,DPPH radical scavenging test and reducting power.The content of isolithospermic acid was 98.7％by normalization method,and the silica gel column chromatography can not separate it completely.The IC50 of isolithospermic acid to OH and DPPH were 0.277 mg·mL-1 and 17.43 μg·mL-1,the antioxidant activity was dose-dependent.The method which combined with MCI-Gel、LH-20 gel and C18 bonded packing column chromatography can be used for the separation of isolithospermic acid,and the isolithospermic acid had good antioxidant activity in vitro,it could be used for the development of cerebrovascular disease drugs.
isolithospermic acidisolation and purificationantioxidant activity
万方数据
维普
