Simultaneous Determination of Caffeic Acid,Astragulin and Potengriffioside A in Extract of Duchesnea Indica(Andr.)Focke
To establish a method for determination of caffeic acid,astragulin and potengriffioside A in extract of Duchesnea indica(Andr.)Focke.HPLC method was used for gradient elution of Hypersil ODS-C18(250×4.6 mm,5 μm),acetonitrile(A)-0.15%trifluoroacetic acid solution(B).Detection wavelength:330 nm;flow rate:1 mL·min-1;Column temperature 35℃.Good linear relationship were found in the the range of 5.91-236.4 μg·mL-1 for caffeic acid,4.18-167.2 μg·mL-1 for astragulin,3.548-141.9 μg·mL-1 for potengriffioside A.Their average recoveries were 98.19%,98.99%and 99.00%,respectively.The method is accurate,convenient and highly operable,and can be used for the quality control and evaluation of extract of Duchesnea indica(Andr.)Focke.
extract of Duchesnea indica(Andr.)Fockecaffeic acidastragulinpotengriffioside Acontent determination
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