Effects of rice straw returning on the community structure and diversity of nitrogen-fixing gene (nifH) in paddy soil
Taking a long-term fertilization experiment in Taoyuan Ago-ecosystem Research Station under Chinese Academy of Sciences as the platform,and selecting four treatments (no fertilization,CK; rice straw returning,C ; nitrogen,phosphorus and potassium fertilization,NPK ; and NPK+C) as the objects,soil samples were collected at the tillering,booting and maturing stages of rice,and the abundance,composition and diversity of nifH-containing bacterial community were measured by real-time quantitative PCR and terminal restriction fragment length polymorphism (T-RFLP),aimed to understand the effects of rice straw returning on the nifH-containing bacterial community in paddy soil.Compared with CK,treatments NPK+C and NPK increased the abundance of nifH-containing microorganisms significantly (except at tillering stage),and NPK+C had the highest abundance of nifH-containing microorganisms.Under the effects of long-term fertilization,the composition of nifH gene community in CK differed obviously from that in the other three treatments.The nifH composition had definite difference between C and NPK,but less difference between NPK and NPK+C.Long-term fertilization did not induce significant changes in nifH diversity.Therefore,long-term rice straw returning not only induced the changes of nifH gene community composition,but also resulted in a significant increase in the abundance of nifH-containing community,and hence,the increase of soil nitrogen fixing capacity.
国家科技期刊平台
NSTL
万方数据
维普
