首页|甲烷及三氯乙烯驯化对垃圾填埋场覆盖土细菌群落结构的影响

甲烷及三氯乙烯驯化对垃圾填埋场覆盖土细菌群落结构的影响

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
对典型垃圾填埋覆盖土进行CH4原位富集和三氯乙烯(TCE)驯化，研究了其生物氧化能力和微生物群落结构变化。覆盖土CH4氧化速率为0。20～0。87 μmol·g-1soil·h-1，TCE降解速率为0。009～0。013 mg·L-1·h-1，其中山东垃圾填埋场覆盖土土样甲烷氧化活性高于广东、上海和重庆地区土样。通过Illumina MiSeq测序技术分析了α多样性和驯化前后微生物菌群结构变化规律。结果表明:在所有被注释的操作分类单元聚类结果中，细菌OTUs分配为39个门，85个纲，562个属，富集驯化后变形杆菌门、拟杆菌门、绿弯菌门和酸杆菌门仍为各土样的优势菌群，所占比例之和高于77。4％;γ-变形杆菌纲、β-变形杆菌纲、α-变形杆菌纲、放线菌纲和酸杆菌纲所占比例之和高于26。5％。嗜甲基菌属、厌氧绳菌属、节杆菌属和假单胞菌属经TCE驯化后，其相对丰度呈增加趋势。表明在覆盖土氯代烃生物降解过程中，除了被广泛认可的甲烷氧化菌异养共代谢机制以外，还存在非甲烷共代谢机制和氯代烃自养降解机制。
Influence of methane and trichloroethylene domestication on bacterial community structure in landfill cover soil
The biological oxidation capacity and change of microbial community structure of typical landfill cover soil cultured in situ by methane and trichloroethylene (TCE) were studied.The rates of CH4 oxidation and TCE degradation were 0.20-0.87 μmol · g-1soil · h-1 and 0.009-0.013 mg · L-1 · h-1 among these landfill cover soils,respectively.The activity of CH4 oxidation in Shandong landfill cover soil was higher than that in landfill cover soils sampled from Guangdong,Shanghai and Chongqing.The α-diversity and microbial community structure before and after co-metabolism degradation were then investigated in all landfill cover soils by Illumina MiSeq sequencing.The results indicated that bacterial Operational Taxonomic Units (OTUs) were assigned to 39 phyla,85 classes and 562 genera in all the commented OTUs clustering.The dominant bacterial populations were Proteobacteria,Bacteroidetes,Chloroflexi and Acidobacteria and the sum of the proportion was over 77.4％ in all landfill cover soils.In addition,total percent of γ-Protebacteria,β-Protebacteria,α-Proteobacteria,Actinobacteria and Acidobacteria was over 26.5％.Specifically,the relative abundances of Methylophilaceae_uncultured,Anaerolineaceae_uncultured,Arthrobacter and Pseudomonas increased obviously after acclimated by TCE.These results suggested that there were cometabolism degradation with non-methane compounds and aerobic assimilation of TCE besides the widely-agreed co-metabolism degradation by methanotrophs.

landfill cover soiltrichloroethyleneIllumina MiSeq sequencingbacterial community structure

赵天涛、何芝、张丽杰、邢志林、高艳辉、彭绪亚

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重庆理工大学化学化工学院,重庆400054

重庆大学城市建设与环境工程学院,重庆400030

重庆理工大学药学与生物工程学院,重庆400054

垃圾填埋场覆盖土 三氯乙烯(TCE) Illumina MiSeq测序 微生物群落结构

国家自然科学基金国家自然科学基金重庆市基础与前沿研究项目重庆理工大学研究生创新基金

5137852241502328cstc2015jcyjB0015YCX2015229

2017

10.13287/j.1001-9332.201705.012

应用生态学报
中国生态学学会 中国科学院沈阳应用生态研究所

应用生态学报

CSTPCDCSCD北大核心
影响因子：2.114
ISSN：1001-9332
年,卷(期)：2017.28(5)
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