Microorganisms are essential actors in the biogeochemical cycling of elements within terrestrial ecosys-tems,with significant influences on soil health,food security,and global climate change.The contribution of microbial anabolism-induced organic compounds is a non-negligible factor in the processes associated with soil car-bon(C)storage and organic matter preservation.In recent years,the conceptual framework of soil microbial carbon pump(MCP),with a focus on microbial metabolism and necromass generation process,has gained widespread attention.It primarily describes the processes of soil organic C formation and stabilization driven by the metabolic activities of soil heterotrophic microorganisms,representing an important mechanism and a focal point in current research on terrestrial C sequestration.Here,we reviewed the progress in this field and introduced the soil MCP conceptual framework 2.0,which expands upon the existing MCP model by incorporating autotrophic microbial pathway for C sequestration and integrating the concept of soil mineral C pump.These advancements aimed to enrich and refine our understanding of microbial-mediated terrestrial ecosystem C cycling and sequestration mecha-nisms.This refined framework would provide theoretical support for achieving China's"dual carbon"goals.