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外源6-BA对孕穗期低温胁迫后小麦旗叶生理、产量及品质的影响

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春季低温通常发生在冬小麦孕穗期,导致小麦大幅减产。本研究选用低温反应敏感型小麦品种'皖麦52'和低温反应迟钝型小麦品种'烟农19'作为试验材料,在孕穗期将小麦盆栽置于人工气候室内进行低温处理(-2和0℃),低温处理结束后分别喷施浓度为10、20、30 mg·L-1的6-苄氨基腺嘌呤(6-BA)溶液,以喷施等量蒸馏水作为对照,探讨孕穗期低温胁迫后喷施6-BA对小麦旗叶部分生理特性、产量和品质的影响。结果表明:与对照相比,外源喷施6-BA处理的小麦幼穗形态较为饱满,小花形态得到改善,穗下节间维管束数目增加;6-BA促进了小麦旗叶可溶性糖、可溶性蛋白和脯氨酸的积累;过氧化物酶和超氧化物歧化酶活性提高,丙二醛含量降低。外源喷施6-BA后小麦退化穗数减少,穗粒数和千粒重提高,籽粒蛋白质、湿面筋含量和沉降值增加。综上,外源喷施6-BA可以有效缓解低温胁迫对小麦旗叶和产量造成的影响。在本试验条件下,喷施6-BA溶液对烟农19的缓解效果要高于皖麦52,其中以喷施20 mg·L-1的6-BA溶液对低温胁迫的缓解效果最好。
Effects of exogenous 6-BA on flag leaf physiology,yield,and quality of wheat after low temperature stress at booting stage
Low temperature(LT)in spring usually occurs at the booting of winter wheat,resulting in reduction of wheat yield.In this study,we used the LT-sensitive wheat cultivar'Wanmai 52'and the LT-insensitive wheat cul-tivar'Yannong 19'as experimental materials to conduct LT treatment(-2 ℃ and 0 ℃)at booting stage.After the LT treatment,we sprayed 6-benzylaminoadenine(6-BA)solutions with concentrations of 10,20,and 30 mg·L-1 respectively,with equal mass distilled water as control to investigate the effects of spraying 6-BA on the physiologi-cal characteristics,yield and quality of wheat flag leaves after LT stress at booting stage.The results showed that compared with the control,young ear of wheat treated with exogenous spraying 6-BA was fuller,the floret morphol-ogy was improved,and the number of vascular bundles under the spike was increased.6-BA application promoted the accumulation of soluble sugar,soluble protein,and proline in flag leaves.The activities of peroxidase and su-peroxide dismutase were increased,and the content of malondialdehyde was decreased.Exogenous 6-BA application decreased the number of degenerated spikes of wheat,increased the number of grains per spike and 1000-grain weight,as well as the contents of grain protein,wet gluten,and sedimentation value.In summary,exogenous 6-BA application could effectively alleviate the effects of LT stress on flag leaf and yield of wheat.Under the conditions of this experiment,the mitigation effect of spraying 6-BA solution on Yannong 19 was higher than that of Wanmai 52,and the mitigation effect of spraying 20 mg·L-1 6-BA solution on low temperature stress was the best.

wheatlow temperature at bootingantioxidant enzyme activityyieldquality

周琦锐、赵梦停、杨丽、栾佳萌、高源、黄正来、马尚宇、樊永惠、张文静

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安徽农业大学农学院,合肥 230036

小麦 孕穗期低温 抗氧化酶活性 产量 品质

国家自然科学基金项目"十四五"国家重点研发计划项目安徽省高等学校科学研究项目安徽省大学生创新创业计划项目

323722232022YFD2301404-52023AH040133S202210364136

2024

应用生态学报
中国生态学学会 中国科学院沈阳应用生态研究所

应用生态学报

CSTPCD北大核心
影响因子:2.114
ISSN:1001-9332
年,卷(期):2024.35(6)