Low temperature(LT)in spring usually occurs at the booting of winter wheat,resulting in reduction of wheat yield.In this study,we used the LT-sensitive wheat cultivar'Wanmai 52'and the LT-insensitive wheat cul-tivar'Yannong 19'as experimental materials to conduct LT treatment(-2 ℃ and 0 ℃)at booting stage.After the LT treatment,we sprayed 6-benzylaminoadenine(6-BA)solutions with concentrations of 10,20,and 30 mg·L-1 respectively,with equal mass distilled water as control to investigate the effects of spraying 6-BA on the physiologi-cal characteristics,yield and quality of wheat flag leaves after LT stress at booting stage.The results showed that compared with the control,young ear of wheat treated with exogenous spraying 6-BA was fuller,the floret morphol-ogy was improved,and the number of vascular bundles under the spike was increased.6-BA application promoted the accumulation of soluble sugar,soluble protein,and proline in flag leaves.The activities of peroxidase and su-peroxide dismutase were increased,and the content of malondialdehyde was decreased.Exogenous 6-BA application decreased the number of degenerated spikes of wheat,increased the number of grains per spike and 1000-grain weight,as well as the contents of grain protein,wet gluten,and sedimentation value.In summary,exogenous 6-BA application could effectively alleviate the effects of LT stress on flag leaf and yield of wheat.Under the conditions of this experiment,the mitigation effect of spraying 6-BA solution on Yannong 19 was higher than that of Wanmai 52,and the mitigation effect of spraying 20 mg·L-1 6-BA solution on low temperature stress was the best.
wheatlow temperature at bootingantioxidant enzyme activityyieldquality
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