Revealing nutrient limitation status of microorganisms in the soil of Robinia pseudoacacia plantation through soil stoichiometry and enzyme metrology
Exploring nutrient limitation in forest soil holds significant implications for forest tending and management.However,current research on nutrient limitation status of microorganisms in Robinia pseudoacacia plantations within the Loess Plateau remains insufficient.To investigate soil microbial nutrient limitation of R.pseu-doacacia plantations on the Loess Plateau,we selected R.pseudoacacia plantations with different afforestation time series(15,25,35,and 45 years)and a pile of barren slope cropland(control)in Yongshou County,Shaanxi Province as the research objects.We analyzed the contents of soil organic matter,total nitrogen,and total phos-phorus,and the activities of β-1,4-glucosidase(BG),cellobiose hydrolase(CBH),leucine aminopeptidase(LAP),β-1,4-N-acetylglucoside(NAG)and phosphatase(AP).We analyzed the soil nutrient limitation by stoi-chiometry and enzyme metrology.The results showed a shift in soil pH from alkaline to acidic during vegetation res-toration process,and that total phosphorus exhibited a gradual decrease over the course of 0 to 25 years.Soil orga-nic matter,total nitrogen and enzyme activities exhibited an increasing trend during the same time frame.However,between 25 and 45 years of age,soil total phosphorus,soil organic matter,total nitrogen,AP and LAP gradually declined while NAG,BG,and CBH initially increased and then decreased.Notably,the values of(BG+CBH)/(LAP+NAG),(BG+CBH)/AP and(LAP+NAG)/AP in R.pseudoacacia plantations were higher than the global average throughout the process of vegetation restoration.In the study area,the vector length was less than 1 and gradually increased,indicating that a progressive increase in microbial carbon limitation during the process of vege-tation restoration.The vector angle exceeded 45° and exhibited an overall decreasing trend,suggesting that soil microorganisms were constrained by phosphorus(P)with a gradual deceleration of P limitation,without any nitro-gen(N)limitation.The restoration of R.pseudoacacia plantation resulted in significant change in soil physical and chemical properties,while the time series of afforestation also influenced nutrient limitation of soil microorganisms.
万方数据
维普
