不同运动状态下胰岛素对2型糖尿病大鼠骨髓间充质干细胞分化的影响
Effects of Insulin on BMSCs Differentiation in Type 2 Diabetic Rats under Different Exercise Conditions
武晓丹 1宫赫 1史佩佩 1胡小容1
作者信息
- 1. 北京航空航天大学 生物与医学工程学院;生物力学与力生物学教育部重点实验室;北京市生物医学工程高精尖创新中心,北京 100083
- 折叠
摘要
目的 研究正常活动和剧烈运动下胰岛素治疗对 2 型糖尿病大鼠股骨远端松质骨中固体受力和流体流动特性、骨髓间充质干细胞(bone marrow stromal cells,BMSCs)分化的影响.方法 基于Micro-CT扫描图像构建正常活动和剧烈运动下 4 周和 8 周胰岛素治疗实验的对照组、糖尿病组、治疗组和安慰剂组大鼠股骨远端松质骨和流体的有限元模型,并利用流固耦合数值模拟方法,分析各组模型的力学参数和细胞分化参数;并对力学参数、细胞分化参数与形态学参数进行相关性分析.结果 正常活动和剧烈运动下,胰岛素治疗改善了 2 型糖尿病大鼠的固体和流体力学参数、BMSCs分化参数.对于 4 周实验,正常活动与剧烈运动下胰岛素治疗分别使 2 型糖尿病大鼠的骨分化面积从 64.024%增加至 69.372%,以及从 73.225%增加至 75.336%;对于 8 周实验,正常活动与剧烈运动下胰岛素治疗分别使 2 型糖尿病大鼠的骨分化面积从 67.239%增加至 72.910%,以及从 76.147%增加至 78.291%.形态学参数中的BV/TV、Tb.N、Tb.Th、Tb.Sp、SMI与骨、软骨分化面积均具有显著相关性(P<0.05).结论 剧烈运动下 8 周胰岛素治疗组松质骨表面的BMSCs更容易分化形成骨组织.研究结果 对进一步理解正常活动和剧烈运动下胰岛素对骨的影响具有重要意义,并为临床上治疗 2 型糖尿病时胰岛素治疗周期和运动方式的选择提供理论指导.
Abstract
Objective To investigate the effects of insulin therapy on the mechanical behavior of solids,characteristics of fluid flow,and bone marrow stromal cells(BMSCs)differentiation in the distal femoral cancellous bone of type 2 diabetic rats under normal activity and vigorous exercise conditions.Methods The finite element models of cancellous bones and fluids in the distal femurs of rats in the control,diabetes,treatment,and placebo groups in 4-week and 8-week insulin treatment experiments under normal activity and vigorous exercise conditions were established based on micro-CT scanning images.The mechanical and cell differentiation parameters of the models in each group were analyzed using the fluid-solid interaction numerical simulation method.Correlations between mechanical,cell differentiation,and microstructural morphology parameters were also analyzed.Results Insulin therapy under normal activity and vigorous exercise conditions improved the solid and fluid mechanical parameters and BMSC differentiation parameters in type 2 diabetic rats.In the 4-week experiment,insulin treatment under normal activity and vigorous exercise conditions increased the differentiation areas of bone in type 2 diabetic rats from 64.024%to 69.372%and from 73.225%to 75.336%,respectively;in the 8-week experiment,insulin treatment under normal activity and vigorous exercise conditions increased the differentiation areas of bone in type 2 diabetic rats from 67.239%to 72.910%and from 76.147%to 78.291%,respectively.Morphological parameters BV/TV,Tb.N,Tb.Th,Tb.Sp,and structure model index were significantly correlated with the differentiation areas of the bone and cartilage(P<0.05).Conclusions Under vigorous exercise conditions,BMSCs on the surface of cancellous bone in the 8-week insulin treatment group were more likely to differentiate into bone tissue.This study is of great significance for further understanding the effects of insulin on the bone under normal activity and vigorous exercise conditions,and provides theoretical guidance for the selection of the insulin therapy cycle and exercise mode in the clinical treatment of type 2 diabetes.
关键词
2/型糖尿病/胰岛素/松质骨/流固耦合/流体剪应力Key words
type 2 diabetes/insulin/trabecular bone/fluid-solid interaction/fluid shear stress引用本文复制引用
出版年
2024
NETL
NSTL
万方数据