轻度创伤型大鼠颅脑前后向-侧向碰撞HIC值等效研究
HIC Value of Mild Traumatic Rats under Anterior-Posterior and Lateral-Medial Craniocerebral Impact:An Equivalent Study
王国祥 1朱林娜 1王讯 1陈秋菊 1熊涛 1罗庆航 1于佳 1许竞毓 1尹志勇 2刘盛雄1
作者信息
- 1. 重庆理工大学 药学与生物工程学院,重庆 400054
- 2. 中国汽车工程研究院股份有限公司,重庆 401122
- 折叠
摘要
目的 探讨通过运动诱发电位(motor evoked potential,MEP)和 β-淀粉样前体蛋白(β-amyloid precursor protein,β-APP)免疫组化(immunohistochemistry,IHC)互相验证结合的方法,对轻度颅脑损伤下大鼠颅脑前后向-侧向碰撞损伤进行头部损伤指标(head injury criterion,HIC)等效转换研究.方法 健康成年雄性SD大鼠 60 只,随机分为 0 m对照组、0.5 m前后向和 0.5 m侧向损伤组、1 m前后向和 1 m侧向损伤组(每组 12 只),对照组不进行打击实验.IHC组在打击致伤后和对照组一起进行过量麻醉取材,制作β-APP免疫组化染色切片,并测定切片的脑干锥体束区域内的阳性细胞面积占比和积分光密度(integral optical density,IOD).MEP组分组和IHC组相同,在致伤后和对照组一起进行MEP 波幅检测.结果 随致伤高度的增加,致伤组MEP 下降幅度、阳性细胞面积占比及IOD也明显增大.致伤高度较小时,IHC组比MEP 组的灵敏度更高.损伤程度相同时,侧向HIC小于前后向.HIC相同时,侧向的损伤程度大于前后向.结论 利用MEP和β-APP联合评估可以为颅脑前后向-侧向碰撞损伤HIC等效转换研究提供实验参考.
Abstract
Objective To investigate the equivalent conversion of head injury criterion(HIC)under anterior-posterior(AP)and lateral-medial(LM)craniocerebral impact for mild craniocerebral injury in rats using motor evoked potential(MEP)and β-amyloid precursor protein(β-APP)immunohistochemistry(IHC).Methods Sixty healthy adult male SD rats were randomly divided into 0 m control group,0.5 m-AP and 0.5 m-LM injury groups,and 1 m-AP and 1 m-LM injury groups(12 rats in each group).The control group did not undergo any impact injury experiment.After the impact injury experiment,the injury and control groups were subjected to excessive anesthesia to produce β-APP immunohistochemical stained slices,and the percentage of positive area and integral optical density(IOD)in the brainstem pyramidal tract area of the slices were determined.The MEP groups were divided in the same manner as the IHC groups and the MEP amplitudes of the MEP and control groups were measured after the impact injury experiment.Results With an increase in the degree of injury,the decrease in MEP amplitude,percentage of positive areas,and IOD in the injury groups significantly increased.When the degree of injury was low,the sensitivity of IHC was higher than that of MEP.When the degree of injury was the same,the HIC in the LM direction was lower than that in the AP direction.When the HIC was the same,the degree of injury in the LM direction was greater than that in the AP direction.Conclusions The joint evaluation of MEP and β-APP can provide experimental references for the study of HIC equivalent conversion in AP-LM craniocerebral impact injury.
关键词
组织病理学/运动诱发电位/脑干锥体束/头部损伤指标/颅脑碰撞Key words
histopathology/motor evoked potential/brainstem pyramidal tract/head injury criterion/craniocerebral impact引用本文复制引用
基金项目
国家重点研发计划(2016YFC0800702-3)
重庆理工大学研究生教育高质量发展行动计划(gzlcx20222091)
出版年
2024
NETL
NSTL
万方数据