目的 研究发育性髋关节发育不良(developmental dysplasia of the hip，DDH)力学负荷异常对软骨下骨的骨显微结构和骨血管的影响，以及骨显微结构和骨血管之间的相关性。方法 使用襁褓法构建新生大鼠DDH模型，对股骨头的关节软骨和软骨下骨进行苏木素-伊红染色、番红固绿染色和免疫组化染色等组织学染色，对软骨下骨进行显微计算机断层扫描(Micro-CT)分析骨显微结构。结果 DDH大鼠出现关节软骨退变，同时伴有软骨下骨的骨显微结构恶化、骨形成减少和血管形成增多，且软骨下骨的血管形成水平与骨显微结构恶化程度呈正相关。结论 DDH力学负荷异常导致关节软骨出现退变，软骨下骨的血管形成增多，软骨下骨的骨显微结构恶化，相关性分析揭示了软骨下骨的血管异常形成可能是造成骨显微结构恶化、DDH进展的重要原因。本研究为探究DDH疾病的发生发展提供了新的研究方向。
Effects of Subchondral Bone Vascularization on Bone Microstructure in Developmental Hip Dysplasia
Objective To study the effects of an abnormal mechanical load in the developmental dysplasia of the hip(DDH)on the bone microstructure and blood vessels of the subchondral bone,and the correlation between bone microstructure and blood vessels.Methods A newborn rat DDH model was constructed using the swaddling method.Histological staining such as hematoxylin-eosin staining,safranin-fast green staining,and immunohistochemical staining was performed on the articular cartilage and subchondral bone of the femoral head.The microstructure of the subchondral bone was analyzed using microcomputed tomography(micro-CT).Results The DDH rats showed degeneration of the articular cartilage accompanied by deterioration of the microstructure of the subchondral bone,decreased bone formation,and increased vascular formation.The level of vascular formation in the subchondral bone was positively correlated with the degree of deterioration of the bone microstructure.Conclusions An abnormal mechanical load in the DDH causes articular cartilage degeneration,increased vascular formation in the subchondral bone,and subchondral bone microstructure deterioration.The correlation analysis revealed that the abnormal vascular formation in the subchondral bone may be an important factor causing the deterioration of bone microstructure and progression of DDH.This study has provided a new direction for the development of DDH.
developmental dysplasia of the hip(DOH)mechanical loadsubchondral boneangiogenesisbone microstructure
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